There were no missing or excluded values, and the exact number of animals used for each experiment and all raw data generated or analysed are provided in the individual figures legends and the Supplementary Raw data file, respectively.References Tidball, J. G. Regulation of muscle growth and ...
Supplementary Figure Legends Table S1 Table S2 Figure S1 Figure S2 Figure S3 Figure S4 Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or forma...
Experimental replicates are mentioned in relevant figure legends. Transfections were performed at least three times, each in triplicate. Statistical analysis was performed using one-way analysis of variance and Student's t test. p values of p < 0.05 (*) were considered as significant. Error bars...
they were reported as a range. The exact sample size (N) for each comparison group was provided in the figure and/or the legends. All the GRID-seq and BL-HiChIP libraries
Myotubes or gastrocnemius samples were lysed with cold RIPA buffer (Beyotime Biotechnology, Shanghai, China) containing 1 mM NaF, 1 mM sodium orthovanadate, and 1 mM phenylmethylsulfonyl fluoride. An equal amount of protein was separated by 4-20% SDS-PAGE (GenScript Biotechnology, Nanjing...
Briefly, total RNA (1 µg) was retro-transcribed using the Script Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). RT-qPCR was performed using the g SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). All ...
Please find more details about the numbers in the figure legends. For co-expression experiments in 10T1/2 cells, two indices were used to examine fusion efficiency. The first fusion index (Fig. 7c) was based on the experiment shown in Supplementary Fig. 7b, and was calculated as the ...
s test was used to compare the difference among three or more groups. All statistical analyses were performed with SPSS 19.0 (Chicago, IL, USA) and GraphPad Prism 5 software (La Jolla, CA). In figure legends, n refers to number of samples. All experimental groups contain at least three ...
Abstract Experimental cell therapies for skeletal muscle conditions have shown little success, primarily because they use committed myogenic progenitors rather than true muscle stem cells, known as satellite cells. Here we present a method to generate in vitro-derived satellite cells (idSCs) from skele...