even when normalization to body weight was conducted (Supplementary Fig.1a–e). Gene-expression analysis by qPCR in different skeletal muscles showed increased expression of atrophy-related genes (MAFbx/Atrogin-
The diaphragm is a unique skeletal muscle due to its continuous activation pattern during the act of breathing. The ontogeny of macrophages, pivotal cells for skeletal muscle maintenance and regeneration, is primarily based on two distinct origins: postn
P values are indicated in figure legends. Real-time RT-PCR data between conditions were analyzed using the non-parametric Mann-Whitney test. For comparison of lamellipodia formation data were analyzed using a linear model with fixed conditions (siDIAPH3/siControl, PDGF+/Vehicle, Rater, ...
they were reported as a range. The exact sample size (N) for each comparison group was provided in the figure and/or the legends. All the GRID-seq and BL-HiChIP libraries
Myotubes or gastrocnemius samples were lysed with cold RIPA buffer (Beyotime Biotechnology, Shanghai, China) containing 1 mM NaF, 1 mM sodium orthovanadate, and 1 mM phenylmethylsulfonyl fluoride. An equal amount of protein was separated by 4-20% SDS-PAGE (GenScript Biotechnology, Nanjing...
Briefly, total RNA (1 µg) was retro-transcribed using the Script Reverse Transcription Supermix (Bio-Rad, Hercules, CA, USA). RT-qPCR was performed using the g SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad). All ...
Supplementary Figure Legends Table S1 Table S2 Figure S1 Figure S2 Figure S3 Figure S4 Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or forma...
s test was used to compare the difference among three or more groups. All statistical analyses were performed with SPSS 19.0 (Chicago, IL, USA) and GraphPad Prism 5 software (La Jolla, CA). In figure legends, n refers to number of samples. All experimental groups contain at least three ...
nuclease-free water and allowed to dissolve at 4 °C for 1 h before being stored at −20 °C. First, strand cDNA synthesis was performed using iScript RT Supermix from Bio-Rad (Saint-Laurent, QC, Canada) according to the manufacturer's instructions. All primers used are appended...
Experimental cell therapies for skeletal muscle conditions have shown little success, primarily because they use committed myogenic progenitors rather than true muscle stem cells, known as satellite cells. Here we present a method to generate in vitro-de