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In addition, taking into account the issue of multiple testing, A false discovery rate (FDR) correction was performed using the online tool Bioladder. An FDR-adjusted P value < 0.05 indicates a statistically significant causal relationship. Finally, funnel plots and scatter plots were utilized...
A PageRuler Plus pre-stained protein ladder was included for reference (Thermo Fisher, 26619). The gel was transferred to a nitrocellulose membrane at 30 V for 1 h. The membrane was blocked in 5% nonfat milk in PBS with 0.05% Tween 20 for 1 h at RT before incubating with primary ...
Hence, future work will examine resistance (e.g., ladder climbing) and/or higher intensity training (e.g., treadmill running) regimens, amongst others. Physical rehabilitation can start within days or weeks following surgery. Initiation of wheel running one week post-injury during the early ...
Population Fact Sheets: World, Available at: https://gco.iarc.fr/today/data/factsheets/populations/900-world-fact-sheets.pdf; 2018 [accessed 27 October 2020]. Google Scholar [2] European Commission. Estimates of cancer incidence and mortality in 2020, for all cancer sites, Available at: ...
Chemiluminescence signal and protein ladder images were acquired using ChemiDoc Imaging System (BioRad). For filter trap, sonicated protein fractions were treated with 100 mM DTT or deionized water for 30 min on ice and then diluted in PBS containing 1% SDS (PBS-SDS). Final protein ...
Intact genomic DNAs of MDG/TRPC3 KD myoblasts on D0 were started to changing to a regularly spaced ladder pattern on D3 by internucleosomal DNA fragmentations (indicated by short bars in the right panel). Wild-type myoblasts or myotubes on the corresponding days were used as positive ...
The size of the ladder was shown on the right. (d) The expression of Malat1 and Myogenin during C2C12 differentiation was examined by qRT-PCR. (e) The expression of Malat1 and Myogenin in early-differentiating satellite cells freshly isolated from mouse limb muscles. (f) The expression of ...
The biotinylated protein ladder detection pack was from Cell Signaling Technology, Inc (Beverly, MA, USA). The calcineurin assay kit was manufactured by the Nanjing Jiancheng Bioengineering Institute (NJBI; Nanjing, China). FuGENE 6 transfection reagent was obtained from Roche Applied Science. ...