The molecular weights of the immunodetected proteins were verified by using the biotinylated protein ladder (Cell Signaling Technology). Protein band intensity was assessed by scanning densitometry. Arbitrary values were expressed as a percentage of values for untreated C57BL/10 muscle (NaCl-C57), ...
The size of the ladder was shown on the right. (d) The expression of Malat1 and Myogenin during C2C12 differentiation was examined by qRT-PCR. (e) The expression of Malat1 and Myogenin in early-differentiating satellite cells freshly isolated from mouse limb muscles. (f) The expression of ...
without any added load, in order to reach the rest area at the top of the stairs. The procedure was repeated six times per session, three sessions a week on alternate days (adapted from [24]).
Lanes in B are as in A, except that lane 6 is a molecular mass ladder, in base pairs (bp). Amplification of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) served as positive control for RT–PCR reactions. 4. Discussion The primary objective of this study was to test the hypothesis that...
(except for mitochondrial complexes), muscle lysates were loaded with Protein Ladder One Plus (19,593–25, Nacalai Tesque) in NuPAGE 3–8% Tris–Acetate gels (NW04122BOX, Thermo Fisher Scientific) for dystrophin detection or in Bolt 4–12% Bis–Tris Plus gels (EA0375BOX, Thermo Fisher ...
Chemiluminescence signal and protein ladder images were acquired using ChemiDoc Imaging System (BioRad). For filter trap, sonicated protein fractions were treated with 100 mM DTT or deionized water for 30 min on ice and then diluted in PBS containing 1% SDS (PBS-SDS). Final protein ...
A G+A sequencing ladder (not shown) run in parallel with the footprinting reactions was used to orient the sequence. Deoxyribonuclease I (DNase I) footprinting was used to map the binding site within D4Z4-243. Figure 2C shows that following incubation with nuclear extracts from HeLa cells,...
Between repetitions, the mouse rested for 2 min at the top of the ladder. Grip strength tests were measured using a grip strength meter (DEFII-002, Chatillon, Largo, FL, USA) before and after the intervention. Briefly, after a mice grasped a wire grid with their four limbs, the tail ...