8d), the percentage of EdU-labeled cells (Fig. 8e-f), and a decrease in the expression of genes linked to cell growth (Fig. 8g-i). In contrast, the simultaneous introduction of si-IGF-1 and inhibitor of miR-146a-5p promoted cell growth. Concurrently, following the induction of ...
The fate of the lactate produced is primarily oxidative (55%–70%), with as much as 90% of the labeled carbon in tracer lactate showing up as CO2during active recovery from exercise. Lactate released from muscle enters exclusively oxidative tissues in which lactate is low (e.g., heart, di...
Using the Nanoimager S Mark II microscope (ONI) with 100 × oil-immersion objective, labeled proteins were imaged sequentially at 25, 35, and 50% power for the 640, 561, and 488 nm lasers, respectively, at 1000 frames per channel with the angle of illumination set to 52.5°. Before...
RNN is a powerful learning algorithm inspired by the biological neural networks that constitutes the human brain, and it is trained to present to the network a large number of labeled “examples” [35]. More specifically, long short-term memory (LSTM) neural networks are a widely used type ...
Figure 7.1.Diagram of the human digestive system from the port-of-entry oral cavity to the exit at the anus. The total length of the digestive system in an adult is ~27ft, or 4.5×the average height of 6ft of an adult. The esophagus is ~10 in in length. The small intestine is ~...
A normal ECG tracing is presented in Figure 7. Each component, segment, and interval is labeled and corresponds to important electrical events, demonstrating the relationship between these events and contraction in the heart. There are five prominent points on the ECG: the P wave, the QRS comple...
respectively. Excess fluorescent dyes were washed away after incubating at 37 °C for 30 min in the dark. To investigate cellular uptake, labeled BME or HME particles (5 × 108) were added to each well containing C2C12 cells in a 6-well plate, and their internalization was examined by...
Background The sarcoglycan complex (SC) is part of a network that links the striated muscle cytoskeleton to the basal lamina across the sarcolemma. The SC coordinates changes in phosphorylation and Ca++-flux during mechanical deformation, and these processes are disrupted with loss-of-function mutat...
Figure 1.Major thoracic muscles inDrosophila. (a) Schematic diagram of a midsagittal section showing the right hemithorax of an adult fly. The six dorsolongitudinal muscle (DLM) fibers are labeled a–f and the three dorsoventral muscles (DVM) are labeled in roman numerals. DVM I consists of...
(A) Diagram of 3D muscle construct setup. (B) Representative 3D construct. (C) Whole mount immunostaining of Myosin heavy chain (MyHC). (D) 3D rendering images of MyHC-positive myotubes grown in the constructs. (E) Immunostaining of laminin and MyHC in the transverse section. Laminin-positiv...