cell and often are more highly concentrated near the myoneural, or neuromuscular, junction. The most striking feature of muscle cells viewed under thelight microscopeis their banded appearance. These stripes, orstriations, result from the highly organized arrangement of proteins in the muscle fiber....
a structure that regulates the processing of the GLI family of transcription factors. Here we find that GLI3 processing by the primary cilium plays a critical role for satellite cell function. GLI3 is required to maintain satellite cells in...
The likely reason is that the cells are very close to each other and these lines are not resolvable by the light microscope. We crossed the KAG547 strain into pix-1(gk299374). In this pix-1 nonsense mutant, the muscle cell boundaries clearly have two separated F-actin lines at the ...
Image analysis was performed using ImageJ and Fiji. To determine the percentage of coexpression of multiple PCGs, cells were quantified in triplicate from stacks within defined regions of each of seven zones. Cells were counted using the Cell Counter plugin (http://rsbweb.nih.gov/ij/plugins/...
Zhang et al.“Maturation of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) in 3D collagen matrix: Effects of niche cell supplementation and mechanical stimulation”Acta Biomaterialia(2017) DOI:10.1016/j.actbio.2016.11.058 Zuo, Li et al.“Low Po2 conditions induce reactive o...
type IIA, 200 type IIB, and 60–80 type IIX or IIA/X fibres from the whole PL area were analysed using the same methods. The percentage of each fibre type in PL muscle was analysed by using the Cell Counter plu...
counting each cell separately. For that, the same threshold was applied to each picture using Fiji software. To investigate anisocytosis, the minimum Feret diameter (MinFeret; shortest distance between two parallel tangents of the muscle fiber edges) of the dystrophin-immunolabeled muscle fibers ...
Full size image Muscle biomechanical stiffness Since Col VI levels were higher inA.cahirinusand in view of the role that this molecule plays in regulating the stem cell niche via the muscle biomechanical properties22,23we next examined the physiological stiffness of freshly isolatedM.musculusandA.cahi...
Full size image Production of SASP by senescent cells varies depending on the cell type and inducer29,30. Thus, we selected all the top differentially expressed genes (>1.7-fold) in DM1 and controls samples and we investigated the presence of these genes in the SASP Atlas30, a proteomic dat...
The cell boundaries were outlined with IMAGE software (Scion, Fredrick, MD). The spreading area (i.e., cell projected area) and the perimeter of the cell were measured, and the CSI was calculated from the relationship CSI = 4π· area/(perimeter)2. The CSI assumes values between one (...