staining was performed to detect liver lobule inflammation. Moreover, to characterize the hepatocytes lipid accumulation, bodipy (Sigma) immunofluorescence of primary hepatocytes, HepG2, and AML12 cells was performed. Histological features were observed and captured with a light microscope (OLYMPUS DP80...
Skeletal muscle cell cultureCell differentiationCO2incubatorSmartphone microscopeCell biology educationWatching living cells through a microscope is much more exciting than seeing pictures of cells in high school and college textbooks. However, bringing cell cultures into the classroom is challenging for ...
recent mouse and human skeletal muscle studies pioneered the use of either single-cell RNA sequencing (scRNA-seq)12,13,14,15,16or single-nucleus RNA sequencing (snRNA-seq)17,18,19,20,21to understand muscle cell type heterogeneity
ELECTRON MICROSCOPE STUDIES OF THE MICROVILLI OF HELA CELLS Microvilli of HeLa cells cultured in vitro were preserved for electron microscopic examination at different stages of routine cultivation procedures. By a ... Fisher,W H. - 《Journal of Cell Biology》 被引量: 85发表: 1967年 Effects of...
Illumination (470 nm) was performed on an Axiovert 200 microscope (Zeiss) through a × 20 Fluar objective (numerical aperture: 0.75, Zeiss) with a temperature-controlled LED module (Omicron, LEDMOD LAB 470 nm, Omicron Laserage). Videos were recorded at 60 fps with a CMOS camera (...
We find that programmed cell death-induced muscle fragmentation produces a population of ‘undead’ intermediate cells, which have the capacity to resume proliferation and contribute to muscle regeneration. We demonstrate the derivation of proliferating progeny from differentiated, multinucleated muscle cells...
Skeletal muscle is one of the few mammalian tissues which can rapidly repair itself repeatedly throughout life1,2. Following physical or toxic insults there is a rapid breakdown of the sarcolemma and an infiltration of phagocytic neutrophils and macrophages which ingest the degenerating muscle cell cy...
Briefly, the tissue was embedded in Durcupan, ultrathin sections of 60 nm were cut using an ultramicrotome from Leica (Vienna, Austria), and images were obtained using a Tecnai G2 electron microscope from FEI (Hillsboro, OR, USA). Point counting30 was performed at 6000‐fold magnification...
MicroRNAs are small regulatory noncoding RNAs that repress gene expression at the posttranscriptional level. Previous studies have reported that the expression of miR-23, miR-27, and miR-24, driven from two miR-23–27–24 clusters, is altered by various
In this study, we hypothesise that cell non-autonomous signalling may be important in the communication of lipotoxicity-induced ER stress in skeletal muscle. We demonstrate the presence of lipotoxicity-induced long-chain ceramides secreted in response to lipotoxicity, which function as a paracrine si...