muscle cell, muscle fiber, muscle fibre - an elongated contractile cell that forms the muscles of the body involuntary muscle, smooth muscle - a muscle that contracts without conscious control and found in walls of internal organs such as stomach and intestine and bladder and blood vessels (exclu...
Most previous studies focused on one particular mechanism or cell type, leaving a gap in our understanding of muscle aging as a whole. To address this, recent mouse and human skeletal muscle studies pioneered the use of either single-cell RNA sequencing (scRNA-seq)12,13,14,15,16or single-n...
been identified: myoregulin (MLN) and dwarf open reading frame (DWORF). MLN shares sequence and structural similarity with PLN and SLN and appears to be a major peptide inhibitor of SERCA activity in fast-type skeletal muscle20. By contrast, DWORF represents the only SERCA-activating micropept...
Skeletal Muscle Cell Growth Medium AmsBio #SKM-M medium Fluorescent beads ThermoFischer #F8826 Critical Commercial Assays Click-iT assay Molecular Probes #C1033 or #C10340 RNeasy Micro Kit QIAGEN #74004 miRNeasy Mini Kit QIAGEN #217004 Quant-iT RiboGreen RNA Assay Kit Life Technologies / Thermo...
Callan HG (1955) Recent work on the structure of cell nuclei. In: Fine Structure of Cells. Symposium of the VIIIth Congress in Cell Biology, Leiden 1954, Noordhof, Groningen, pp. 89-109. Google Scholar Callan HG (1982) The Croonian Lecture, 1981. Lampbrush chromosomes. Proc Roy Soc Lon...
astronaut A had negligible expression changes. Other enzymes involved in the cell antioxidant response, such as peroxiredoxin 2, had a pattern of induction at R + 0 that correlated with the different levels of exercise (see Fig.4). Given the established role of exercise in the regulation ...
3D cell culture technology has obvious advantages, but it will be a long process to completely replace 2D cell culture technology because 3D cell culture absolutely requires more funds, complex operation, and experience. Figure 2 Open in figure viewerPowerPoint Schematic diagram of the general ...
Moreover, ~50% of all nuclei within rat skeletal muscle originate from other cell types than muscle fibers8, hence confounding investigations of myofiber-specific proteins. Therefore, we devised a sensitive workflow to obtain the proteome of single muscle fibers9 and subsequently showed that fast ...
In order to further map these sequence data to other structural cell-types of the lung, we then utilized an RNA-Seq lung cell dataset comprising cultured epithelial, endothelial, fibroblast and smooth muscle cells (Amgen, Inc., Thousand Oaks, CA). Three of the ASM de novo identified ORs (...
[58-60]. Further functional analysis is required to validate the predition that the rate of fatty-acid uptake in skeletal muscle peaks during the mid-late inactive period. Upon uptake into the cell, fatty acids can be stored as triglycerides or be converted to acetyl-CoA through β-oxidation...