The GeXP system uses a two-primer endpoint PCR process, with gene-specific universal primers, to run up to two 96-well plates per day. Researchers can test for the expression of about 20 to 30 genes per reaction per well. Althea has offered multiplexed...
一般靶向药都是针对一些已知的基因突变设计的ctdna一般用基于多重pcr扩增的方式捕获需要测的目标序列如果在血浆游离dna两端加上随机的barcode序列然后再进行扩增这样每个游离dna分子经pcr产生的错误或者测序错误就可根据比对到的位置和barcode序列用生物信息学的方法得到校正 分子标签(multiplexedPCR-basedbarcodingofDNA)在...
PCR was performed with the CloneAmp HiFi PCR Premix (Takara #639298) using 30 ng of genomic DNA and target specific primers for each gene (Additional file 1). Gel electrophoresis of the PCR amplicon was gel purified using the QIAquick Gel Extraction Kit (Qiagen #28706) and analyzed by ...
A novel strategy for amplification full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. The amplification of 3′ ends of cDNA is performed according to the modified classic 3′ RACE techniques, therein the more efficient...
Define multiplexed. multiplexed synonyms, multiplexed pronunciation, multiplexed translation, English dictionary definition of multiplexed. adj. 1. Relating to, having, or consisting of multiple elements or parts: "the whole complex and multiplex detail
(microarrays and nanopore sequencing). Here we show that a portable and battery-powered PCR assay performed in a toroidal convection chamber housing a microarray of fluorescently quenched oligonucleotide probes allows for the rapid and sensitive quantification of multiple DNA targets with single-nucleotide...
NASBA可以产生终点结果或实时结果,使其分别类似于PCR和实时PCR。终点NASBA通常伴随着凝胶电泳,该电泳确定核苷酸的大小并检测大小特异性序列。实时NASBA通常更常用,因为它不需要凝胶电泳:它使用分子信标或其他形式的荧光团进行序列检测。荧光团的使用有利于利用光学器件的廉价诊断设备,例如LED和光电二极管。然而,由于缺乏核苷酸...
RT-PCR primer multiplexes and a method for detecting the presence of Subgroup I and II barley or cereal yellow dwarf viruses in a sample. A first multiplex includes a first primer pair that selectively amplifies Subgroup I viruses, and a second primer pair that selectively amplifies Subgroup II...
Oya, Population genetics of 10Y- chromosomal STR loci in Japanese using multiplexed PCR amplification kits, Y-PLEXTM 6 and Y-PLEXTM 5, Int. Con- gress Ser. 1261 (2004) 307-309.A. Kido, M. Hara, H. Kameyama, Y. Yamamoto, R. Susukida, A. Takada, K. Saito, M. Oya, Population ...
Nucleic acid amplification tests (NAATs) are well-suited to the detection of infectious pathogens [25]. The polymerase chain reaction (PCR), for example, can exponentially amplify a small number of nucleic acid fragments specific to particular species or strains of pathogens, including bacteria and...