The Fluidigm Hyperion imaging mass cytometry (IMC) system combines a CyTOF mass cytometer with a laser ablation system allowing for 40+ parameter simultaneous immunophenotyping on a single slide of FFPE tissue, with sub-cellular resolution. Here we describe our efforts to develop and optimize highly...
Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporat
Using this strategy on imaging mass cytometry data, we identified a measurement of tissue spatial segregation that enables optimal experimental design. This strategy will enable an improved design of multiplexed imaging studies.Similar content being viewed by others An end-to-end workflow for ...
The inflammatory infiltrate in skin has not been fully characterized and in this study we took an single cell, unbiased approach by using Imaging mass cytometry (IMC). Substantial monocyte-macrophage diversity was observed with the CD14+ population correlated positively with cutaneous dermatomyositis ...
Imaging Mass Cytometry (IMC™), based on CyTOF®technology, uses antibodies labeled with metal tags for immunostaining samples in a manner analogous to traditional IHC staining. The samples are then introduced into aHyperion™ Imaging Systemfor data acquisition and analysis. “By replacing fluorop...
The growing scale and dimensionality of multiplexed imaging require reproducible and comprehensive yet user-friendly computational pipelines. TRACERx-PHLEX performs deep learning-based cell segmentation (deep-imcyto), automated cell-type annotation (TYPE
质谱流式组织成像(Imaging mass cytometry, IMC)能够对细胞类型和状态进行高维单细胞分析。在大规模细胞术中,稀土金属被用作抗体的报告者。使用质谱细胞仪分析金属丰度可以测定单个细胞中的标志物表达。质谱细胞术以前只应用于细胞悬浮液。为了获得空间信息,作者将免疫组化和免疫细胞化学方法与高分辨率激光消融耦合到CyTOF...
Imaging Mass Cytometry Captures Major Epithelial, Stromal, and Immune Cell Populations in Inflammatory Bowel Disease IMC捕获IBD的主要上皮细胞、间质细胞、免疫细胞群 样本信息:6 CI 末端回肠; 6 CD 末端回肠; 6 CC 结肠; 6 UC 结肠 首先,作者用31种针对结构、上皮和免疫细胞类型的特异性抗体对组织切片进行...
Imaging Mass Cytometry (IMC) is a high-plex spatial biology imaging technique that enables deep characterization of the diversity and complexity of the tumor microenvironment (TME). IMC supports detailed assessment of cell phenotype and function using 40-plus metal-tagged antibodies simultaneously on a...
5A, B). Next, we evaluated a published dataset of Melanoma metastases acquired by Imaging Mass Cytometry (IMC), a different mass-based imaging modality42. We used the cell classifications provided by the authors to train CellSighter on 55 images and tested the results on 16 heldout images. ...