The Fluidigm Hyperion imaging mass cytometry (IMC) system combines a CyTOF mass cytometer with a laser ablation system allowing for 40+ parameter simultaneous immunophenotyping on a single slide of FFPE tissue, with sub-cellular resolution. Here we describe our efforts to develop and optimize highly...
Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporation of the DNA-based signal amplification by exchange...
The inflammatory infiltrate in skin has not been fully characterized and in this study we took an single cell, unbiased approach by using Imaging mass cytometry (IMC). Substantial monocyte-macrophage diversity was observed with the CD14+ population correlated positively with cutaneous dermatomyositis ...
Using this strategy on imaging mass cytometry data, we identified a measurement of tissue spatial segregation that enables optimal experimental design. This strategy will enable an improved design of multiplexed imaging studies.Similar content being viewed by others An end-to-end workflow for ...
Imaging Mass Cytometry (IMC™), based on CyTOF®technology, uses antibodies labeled with metal tags for immunostaining samples in a manner analogous to traditional IHC staining. The samples are then introduced into aHyperion™ Imaging Systemfor data acquisition and analysis. “By replacing fluorop...
The growing scale and dimensionality of multiplexed imaging require reproducible and comprehensive yet user-friendly computational pipelines. TRACERx-PHLEX performs deep learning-based cell segmentation (deep-imcyto), automated cell-type annotation (TYPE
Imaging mass cytometry and multiplatform genomics define the phenogenomic landscape of breast cancer. Nat Cancer. 2020;1:163–75. Article Google Scholar Gerdes MJ, Sevinsky CJ, Sood A, Adak S, Bello MO, Bordwell A, et al. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-...
C. et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332, 687–696 (2011). Article CAS PubMed PubMed Central Google Scholar Giesen, C. et al. Highly multiplexed imaging of tumor tissues with subcellular resolution by ...
Wahlby et al., “Sequential Immunofluorescence Staining and Image Analysis for Detection of Large Numbers of Antigens in Individual Cell Nuclei”, Cytometry, 2002, 47:32-41. Zrazhevskiy et al., “Quantum dot imaging platform for single-cell molecular profiling”, Nat Commun. 2013 4: 1-12...
FIG. 1. Workflow summary of multiplexed ion beam imaging (MIBI). Biological specimens, such as FFPE tissue or cell suspensions, are immobilized on a conductive substrate, such as indium tin oxide coated glass or silicon wafer. Samples are subsequently stained with antibodies conjugated to unique ...