Complex molecular and metabolic phenotypes depict cancers as a constellation of different diseases with common themes. Precision imaging of such phenotypes requires flexible and tunable modalities capable of id
Heinzmann, K., Carter, L. M., Lewis, J. S. & Aboagye, E. O. Multiplexed imaging for diagnosis and therapy.Nat. Biomed. Eng.1, 697–713 (2017). ArticlePubMedGoogle Scholar Wang, Y. W., Reder, N. P., Kang, S., Glaser, A. K. & Liu, J. T. C. Multiplexed optical imaging...
Targeting to the theme of accurate diagnosis and effective therapy of cancer cells, a multifunctional nanoplatform of aptamer and polyethylene glycol (PEG) conjugated MoS2 nanosheets decorated with Cu1.8S nanoparticles (ATPMC) is developed. The ATPMC nanoplatform accomplishes photoluminescence imaging, ...
O. Multiplexed imaging for diagnosis and therapy. Nat. Biomed. Eng. 1, 697–713 (2017). 12. Wang, Y. W., Reder, N. P., Kang, S., Glaser, A. K. & Liu, J. T. C. Multiplexed optical imaging of tumor-directed nanoparticles: A review of imaging systems and approaches. ...
Here, we describe PHLEX, an automated workflow for cell PHenotype and Localisation analysis of multiplEXed imaging (Fig. 1a). Addressing key challenges with IMC data analysis, we developed and integrated three modules in the PHLEX workflow: (i) deep-imcyto for nuclear and whole-cell segmentation...
17 These studies show that although qIHC techniques require further optimization, they are a promising tool to improve cancer diagnosis and therapy. Although qIHC has been evaluated extensively in epithelial cancers, few studies have explored this technology in lymphoma. Chabot-Richards et al.18 ...
In the future, we envision that MIBI-TOF will not only be used for reproducible basic science research, but also enable the adoption of quantitative spatial signatures in the clinic for more accurate diagnosis and therapeutic selection. At the same time, as efforts to develop other IHC-centric ...
Understanding intratumoral heterogeneity—the molecular variation among cells within a tumor—promises to address outstanding questions in cancer biology and improve the diagnosis and treatment of specific cancer subtypes. Single-cell analyses, especiall
In oncology, technologies for clinical molecular imaging are used to diagnose patients, establish the efficacy of treatments and monitor the recurrence of disease. Multiplexed methods increase the number of disease-specific biomarkers that can be detecte
The morphology of the microspheres was evaluated using the EVOS M5000 Imaging System (Invitrogen by Thermo Fisher Scientific, USA), where filters for transmitted light phase-contrast and fluorescence were selected as detectors (Filters: DAPI, Excitation: 357 nm, Emission: 447 nm). Flow cytometry ...