DNA Software™ creates value for its customers by providing more accurate results that significantly save costs and resources required of PCR-based analysis.
Primer design for multiplex PCR using a genetic algorithm - Lin, Huang, et al. - 2005 () Citation Context ...es, the targets should be put to different groups for separate multiplex PCR runs. However, the number of such separate groups should be minimized. There have been many studies ...
PrimerPlexMultiplex PCR Primer Design Software PrimerPlex is an efficient & sophisticated tool for designing primers for multiplex PCR. Multiplex assays facilitate amplification of multiple targets in a single reaction vessel, reducing both, the time and cost of experimentation....
Multiplex PCR Primer Design with PrimerPlexPrimerPlex is an efficient tool to design specific oligos for multiplex PCR assays. The program checks the oligos for cross reactivity and minimizes Tm mismatches to give you the best possible multiplex set. In this process, it analyzes millions of possible...
Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in diagnostic app
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...
multiplex PCR primer pairs using a statistical-based template filter; performs multiple sequence alignment, conserved region identification, and primer design; and evaluates the primers based on template coverage, taxon specificity, and target specificity. PMPrimer identifies conserved regions using Shannon...
SOFTWAREOpenAccessMPprimer:aprogramforreliablemultiplexPCRprimerdesignZhiyongShen1†,WubinQu1†,WenWang1,YimingLu1,YonghongWu1,ZhifengLi1,XingyiHang1,XiaoleiWang2,DongshengZhao2,ChenggangZhang1*AbstractBackground:MultiplexPCR,definedasthesimultaneousamplificationofmultipleregionsofaDNAtemplateormultipleDNAtemplate...
openPrimeR is an R package providing methods for designing, evaluating,and comparing primer sets for multiplex polymerase chain reaction (PCR). The package provides a primer design function that generates novel primer setes by solving a set cover problem such that the number of covered template seq...
Figure 2: Overview of multiplex primer design using Primal Scheme online primer design tool. From: Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples (a) Submission box for online primer design tool. (b) Primer table of results...