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A program named MPprimer was developed to help users for reliable multiplex PCR primer design. It employs the widely used primer design program Primer3 and the primer specificity evaluation program MFEprimer to design and evaluate the candidate primers based on genomic or transcript DNA database, ...
Multiplex PCR is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. In a multiplexing assay, more than one target sequence can be amplified by using multiple primer pairs in a reaction mixture. As an extension to the practical use of PCR, ...
Primer design for multiplex PCR presents several challenges which include primer dimers, inability to separate amplicons with similar electrophoretic mobility and mis-priming due to nonspecific binding to non-target DNA templates. PrimerPlex uses proprietary algorithms to design optimal multiplex primer sets...
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...
What are the challenges of multiplex PCR? Multiplex PCRoffers many advantages, but its initial setup can be challenging due to several factors: Complex Assay Development: Designing specific, non-reactive primers for multiple targets and optimizing reaction conditions (e.g., annealing temperature, cycl...
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Figure 2: Overview of multiplex primer design using Primal Scheme online primer design tool. From: Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples (a) Submission box for online primer design tool. (b) Primer table of results...
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