DNA Software™ creates value for its customers by providing more accurate results that significantly save costs and resources required of PCR-based analysis.
Multiplex PCR is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. In a multiplexing assay, more than one target sequence can be amplified by using multiple primer pairs in a reaction mixture. As an extension to the practical use of PCR, ...
MPprimer: a program for reliable multiplex PCR primer design. BMC Bioinform 2010; 11: 143.Shen ZY,Qu WB,Wang W,et al.MPprimer:a program for reli-able multiplex PCR primer design. BMC Bioinformat . 2010Shen ZY, Qu WB, Wang W, Lu YM, Wu YH, Li ZF, Hang XY, Wang XL, Zhao DS,...
PrimerPlexMultiplex PCR Primer Design Software PrimerPlex is an efficient & sophisticated tool for designing primers for multiplex PCR. Multiplex assays facilitate amplification of multiple targets in a single reaction vessel, reducing both, the time and cost of experimentation....
PanelPlex provides completely automated design of multiplex PCR (Polymerase Chain Reaction) with unprecedented coverage, sensitivity and specificity.
The master mix is based on the high-fidelity Phusion U Hot Start DNA Polymerase, an enzyme developed using fusion protein technology that enables fast, specific, and robust multiplex PCR. The master mix contains all the compone...
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...
What are the challenges of multiplex PCR? Multiplex PCRoffers many advantages, but its initial setup can be challenging due to several factors: Complex Assay Development: Designing specific, non-reactive primers for multiple targets and optimizing reaction conditions (e.g., annealing temperature, cycl...
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