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Primer design for multiplex PCR using a genetic algorithm - Lin, Huang, et al. - 2005 () Citation Context ...es, the targets should be put to different groups for separate multiplex PCR runs. However, the number of such separate groups should be minimized. There have been many studies ...
Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in diagnostic applications of clinical and environmental micro...
DNA methylation at CpG dinucleotides has become a major research focus due to its regulatory role in numerous biological processes, but the requisite need for assays which amplify bisulfite-converted DNA represents a major bottleneck due to the unique design constraints imposed on bisulfite-PCR primer...
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...
openPrimeR is an R package providing methods for designing, evaluating,and comparing primer sets for multiplex polymerase chain reaction (PCR). The package provides a primer design function that generates novel primer setes by solving a set cover problem such that the number of covered template seq...
To develop the rapid diagnosis and typing of human lymphotropic herpesviruses by using multiplex nested-PCR, the primary PCR (1(o) PCR) primers were redesigned as degenerate primers based on a highly conserved sequences of each DNA polymerase gene of EBV, CMV, HHV-6, HHV-7 and HHV-8. ...
Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strat...
Diomni Enterprise Software Real-time PCR Research Solution Diomni Enterprise Software New multiplex qPCR solution helps you get more data from your samples Multiplex qPCR is a simple, efficient, and cost effective solution for overcoming the challenges of limited samples and costly analysis. ...
Design Of Primers For Identification Of Honey Bee Viruses In Multiplex-PCR Astra SalvensisKHAMMADOVA, Alfiya VasilevnaSHURALEV, Eduard ArkadevichKHAMMADOV, Nail IldarovichOUMAROU, Bamanga MamadouFAIZOV, Tagir KhadievichMUKMINOV, Malik Nilovich...