DNA Software™ creates value for its customers by providing more accurate results that significantly save costs and resources required of PCR-based analysis.
Primer design for multiplex PCR presents several challenges which include primer dimers, inability to separate amplicons with similar electrophoretic mobility and mis-priming due to nonspecific binding to non-target DNA templates. PrimerPlex uses proprietary algorithms to design optimal multiplex primer sets...
Primer designMultiplexSoftwareWeb interfaceVariantA large number of variants have been employed in various medical applications, such as providing medication instructions, disease susceptibility testing, paternity testing, and tumour diagnosis. A high multiplicity PCR will outperform other technologies because ...
Multiplex PCR is a widespread molecular biology technique for amplification of multiple targets in a single PCR experiment. In a multiplexing assay, more than one target sequence can be amplified by using multiple primer pairs in a reaction mixture. As an extension to the practical use of PCR, ...
Primer design software So how does one design such compatible primer sets? In a word, software. There are a variety of free calculators available to help with this process, such as Integrated DNA Technologies’ RealTime PCR calculator. Nichols prefersPrimer3(MIT, Cambridge, MA), which she supp...
Multiplex PCRoffers many advantages, but its initial setup can be challenging due to several factors: Complex Assay Development: Designing specific, non-reactive primers for multiple targets and optimizing reaction conditions (e.g., annealing temperature, cycling profile) require significant effort. Mult...
Real-Time PCR Reagents and Kits Diomni Enterprise Software Real-time PCR Research Solution Diomni Enterprise Software Diomni Design and Analysis (RUO) Software for qPCR New multiplex qPCR solution helps you get more data from your samples Multiplex qPCR is a simple, effic...
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...
DNA methylation at CpG dinucleotides has become a major research focus due to its regulatory role in numerous biological processes, but the requisite need for assays which amplify bisulfite-converted DNA represents a major bottleneck due to the unique design constraints imposed on bisulfite-PCR primer...
Real-time and digital PCR instrument support center Real-time and digital PCR applications support center Real-time PCR education center *For Laboratory Use. General purpose reagent. For Research Use Only. Not for use in diagnostic procedures....