Genetic diversity of closely related free-living microorganisms is widespread and underpins ecosystem functioning, but most evolutionary theories predict that it destabilizes intimate mutualisms. Accordingly, strain diversity is assumed to be highly rest
As one round of splitting takes at least 11 days and sometimes results in failure, constructing strains with multiple chromosome splits is very time-consuming and laborious. Thus, a new chromosome splitting technology that enables simultaneous multiple chromosomes splitting would lead to rapid, ...
auris Strains Nineteen strains of C. auris were collected from microbiology labs from the clinical samples of King Fahd Hospital of the University, Khobar, Saudi Arabia and King Fahad Specialist Hospital, Dammam, Saudi Arabia. The clinical isolates were identified by MALDI-TOF MS analysis at the...
In this study, we sequenced the whole genome of seven M. tuberculosis strains, including four extensively drug-resistant (XDR) and two pan-susceptible clinical isolates and M. tuberculosis H37Rv (ATCC 27294), using high-precision PacBio single-molecule real-time (SMRT) sequencing technology [10]...
Similarly, 20 datasets comprised adult individuals from non-Westernized communities (n = 1,195), meaning having limited direct or indirect antibiotics exposure, minimal or none heavily processed food availability, closeness to animals and do not subtend any geographical pattern, and 399 children ...
Escherichia coliis usually a harmless commensal bacteria that colonizes the human gut. However, many different types and strains exist, some of them have virulence properties that can result in inflammation and damage of the prostate. Jain et al. [63] have isolatedE. colifrom benign prostate tis...
represents the 31 kb expected mini-chromosome. The expected mini-chromosome was constructed in two strains (Lane 2 and 8). Four strains are shown to have single splitting at C12-P1 (Lane 3, 4, 6, and 9). Four strains are shown to have single splitting at C12-P2 (Lane 1, 5, 7...
Production of the antigen. The pictures show western blots of cell-free extracts ofAg85B-ESAT6-DC(AgE6-DC) expressing strains harvested 3 hours after induction. Sample sizes were adjusted to the OD600of the harvested culture, meaning that all samples represent approximately equal amounts of ce...
Additionally, when agr-deletion (Δagr) strains are tested in various infection and pathogenesis models in vivo, the bacteria may colonize but disease is attenuated [8–12], and clearance of individual S. aureus cells by host defenses is enhanced [9,13]. We and others are actively ...
Y.Z. designed experiments, analyzed experimental results and wrote the manuscript; J.Z. participated in experiments; L.W. drafted the paper and analyzed data; H.X. participated in microbiology analysis; Z.L. carried out experiments; Y.L. analyzed experimental results; Z.H. participated in des...