Although the function of H2A.Z in euchromatic gene regulation is well known, its function and deposition mechanism in heterochromatin are still unclear. Here, we report that H2A.Z plays multiple roles in fission yeast heterochromatin. While a small amount of H2A.Z localizes at pericentromeric ...
The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast Nat. Cell Biol., 1 (1999), pp. 298-304 View in ScopusGoogle Scholar [28] A.D. Mozdy, J.M. McCaffery, J.M. Shaw Dnm1p GTPase-mediated mitochondrial fission is a multi-step process requiring the novel integral ...
Here, we develop a microscopy-based assay in living fission yeast to determine the dynamics and kinetics of an engineered, site-specific interhomologue repair event. We observe highly efficient homology search and homology-directed repair in this system. Surprisingly, we find that the initial ...
In the fission yeast Schizosaccharomyces pombe the cell integrity MAPK pathway (CIP) regulates morphogenesis, cell wall structure and ionic homeostasis. We show that the Rab GTPase Ryh1, a TORC2 complex activator, cross-activates the CIP and its core member, the MAPK Pmk1, by two distinct ...
RNA polymerase II from the fission yeast Schizo-saccharomyces pombe consists of 12 species of subunits, Rpb1-Rpb12. We expressed these subunits, except Rpb4, simultaneously in cultured insect cells with baculovirus expression vectors. For the isolation of subunit complexes formed in the virus-infecte...
Article Google Scholar Chacko LA, Mikus F, Ariotti N, Dey G, Ananthanarayanan V (2023) Microtubule–mitochondrial attachment facilitates cell division symmetry and mitochondrial partitioning in fission yeast. J Cell Sci 136(1):jcs260705 Article Google Scholar Download references ...
In yeast, caffeine acts on TOR1 unit of TORC1 complex and inactivates it [45]. However, in Dictyostelium TOR is a part of both TORC1 and TORC2 so it is likely that caffeine can inactivate both TORC1 and TORC2. TORC2 regulates chemotaxis and multicellular organization by monitoring the ...
In the fission yeast Schizosaccharomyces pombe, galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases in the lumen of the Golgi apparatus. In S. pombe, the major in vitro ±1,2-galactosyltransferase activity has been purified, the ...
Transient structural variations have strong effects on quantitative traits and reproductive isolation in fission yeast. Nat Commun. 2017;8:14061. Article CAS PubMed PubMed Central Google Scholar Thorvaldsdottir H, Robinson JT, Mesirov JP. Integrative genomics viewer (IGV): high-performance ...
In fission yeast, temperature-sensitive mutations of Pold lead to cell cycle arrest in S phase.4 Furthermore, studies with Xenopus egg extracts have shown that disruption of Pold via immuno- depletion leads to an accumulation of single-stranded DNA gaps, highlighting Pold's role in lagging ...