Here we describe the methods most commonly used to synchronize fission yeast cells.doi:10.1007/978-1-4939-3145-3_20Marta Tormos-PérezLivia Pérez-HidalgoSergio MorenoSpringer New YorkTormos-Perez M, Perez-Hidalgo L, Moreno S. 2016. Fission yeast cell cycle synchronization methods. Methods Mol ...
Fission yeast Cell cycle Initiation of DNA replication Prereplicative complex DNA polymeraseA 4π- β detector was used at the Lohengrin mass separator to ... A Hossain,Y Cui,AE Bolotnikov,... - 《Journal of Electronic Materials》 被引量: 66发表: 1976年 Mitochondrial adenosine triphosphatase ...
Yeasts provide an excellent model system for the study of the basic mechanisms of eukaryotic cell cycle control. Since they are simple eukaryotes, cell cycle regulation is less likely to be confused with the additional controls involved in growth and dif
The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G1 and G2 phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis...
REPORT Cell Cycle 14:6, 848--856; March 15, 2015; Published with license by Taylor & Francis Group, LLC Fission yeast Ryh1 GTPase activates TOR Complex 2 in response to glucose Tomoyuki Hatano1,y, Susumu Morigasaki1,y, Hisashi Tatebe1,y, Kyoko Ikeda2, and ...
Fig. 1. Relative and absolute dynamics of the proteome and phosphoproteome during four phases of the fission yeast cell cycle. Four separate cell cultures of S. pombe representing the cell cycle phases and four separate cell cultures for the super-SILAC standard were labeled using SILAC media con...
Overview The fission yeast Schizosaccharomyces pombe has proved to be a good model system for analysis of the eukaryotic cell cycle. A number of the key cell cycle regulatory molecules now being extensively studied in higher eukaryotes were first identified and characterized in fission yeast, and it...
Supplementary Figure 3 Genome-wide contact maps during the cell cycle. (a) Cells in the different cell-cycle stages were subjected to in situ Hi-C genomic analysis. Contact scores estimated from the in situ Hi-C data were plotted across the fission yeast genome during the cell cycle. (b)...
To identify near complete sets of genes required for the cell cycle and cell shape, we have visually screened a genome-wide gene deletion library of 4843 fission yeast deletion mutants (95.7% of total protein encoding genes) for their effects on these processes. A total of 513 genes have bee...
pombe. Cell 132, 983–995 (2008). CAS PubMed Google Scholar Rustici, G. et al. Periodic gene expression program of the fission yeast cell cycle. Nat. Genet. 36, 809–817 (2004). CAS PubMed Google Scholar Takayama, Y. & Takahashi, K. Differential regulation of repeated histone ...