XYZseq encodes spatial information by physically partitioning tissue sections into an array of microwells containing barcoded primers for reverse transcription primers [35]. The High-Definition Spatial Transcriptomics (HDST) method uses microwell-based fluorescence spatial indexing beads to capture ...
The Amplicon-Seq analysis was performed using Quantitative Insights into Microbial Ecology 2, (QIIME2) (Ref.104;http://qiime.org/). Amplicon sequence libraries were processed, and low-quality ends of reads and primers were trimmed. The forward primer Arc915aF-CS1 ACACTGACGACATGGTTCTACAAGGAATTG...
FLOWERING LOCUS T(FT)28. However, regulators capable of stabilizing MED25 are yet to be discovered. Considering the prominent role of MMC in JA signaling during hormone-induced
Following purification, the RNA was fragmented using divalent cations and first-strand cDNA synthesis carried out using random primers. Second-strand cDNA synthesis was then performed, and the ends of the resulting double-stranded cDNA fragments were repaired, 5′-phosphorylated and 3′-A tailed. ...
to search against the assembled-protein database of CRC. Several highly similar candidate genes were obtained from the BLAST search includingCZG_jg11962(52%),CZG_jg11963(51%),CZG_jg17822(51%),CZG_jg18299(51%),CZG_jg23745(50%) andCZG_jg23753(50%). Gene-specific primers were designe...
USA) and sequencing reactions were performed with forward and reverse M13 primers (5 μM, Life Technologies, Carlsbad, USA) and BigDye(R) Terminator v 3.1 chemistry (Life Technologies, Carlsbad, USA) according to the manufacturer’s instructions using an Applied Biosystems 3730xl DNA Analyzer. ...
The invention discloses a primer combination for guiding the application of a beta-receptor blocker, a multi-gene detection kit and a using method thereof. The kit disclosed by the invention comprises ultrapure water, an X solution, 10*PCR (Polymerase Chain Reaction) buffer, PCR primers, a ...
The Windows® MultiPoint™ Mouse Software Development Kit (SDK) is a development framework that enables you to build applications to support up to 25 mouse devices concurrently on one computer. As a developer, you can use the MultiPoint Mouse SDK to create educational applications for schools...
We remove adapters and primers on the whole read and low-quality nucleotides from both ends (while quality value is lower than 20) and then continue next steps with the longest sequence without adapters and low quality bases. Then we removed sequences between the second unknown nucleotide (N)...
Reactions were incubated on a magnet, the supernatant containing unbound PCR primers and reagents was discarded, and DNA bound SPRI beads were washed twice with 80% v/v ethanol. SPRI beads were resuspended in Buffer EB (Qiagen, 1014609), incubated on a magnet, and the supernatant was ...