With the advancement of modern science and technology, large scientific facilities are increasingly oriented toward demand and application, and can be used for basic research as well as serving multiple disciplines. Developing large scientific facilities
Mitochondrial marker PCR-RFLPCOIXbaI targets the cytochrome oxidase subunit I region with the primers COIXbaF and COIXbaIR, designed to distinguishM. chilensisfrom other mussels. The 233 bp amplicon was restricted with theXbaI enzyme, generating two fragments (134 and 99 bp) inM. chilensis20onl...
Universal primers 338F (5ʹ-ACTCCTACGGAGGCAGCAG-3ʹ) and 806R (5ʹ-GGACTACNNGGGGTATCTAAT-3ʹ) were used to amplify the V3-V4 hypervariable region of 16S rRNA gene of bacteria. Deep sequencing was conducted on the Illumina Miseq/Novaseq (Illumina, Inc., USA) platform at Beijing...
qPCR primers are as follows. • ACTB: Thermo Fisher Scientific, cat no.4331182, assay ID: Hs01060665_g1 • FOXP3: Thermo Fisher Scientific, cat no.4453320, assay ID: Hs01085834_m1 • TNFRSF9: Thermo Fisher Scientific, cat no.4453320, assay ID: Hs00155512_m1 • IL1R1: Thermo ...
In humans, two ubiquitously expressed N-myristoyltransferases, NMT1 and NMT2, catalyze myristate transfer to proteins to facilitate membrane targeting and signaling. We investigated the expression of NMTs in numerous cancers and found that NMT2 levels ar
Brassica napus is an important vegetable oil source worldwide. Seed coat content is a complex quantitative trait that negatively correlates with the seed oil content in B. napus. Here we provide insights into the genetic basis of natural variation of see
Tumor cells from both 2D and 3D MTSs were digested to prepare single-cell suspensions. Oxygraph-2 k mitochondrial function analyzer (Oroboros, Innsbruck, Austria) was used to measure the cellular oxygen consumption rate (OCR) under the following four conditions: routine, normal cell respiration leve...
XYZseq encodes spatial information by physically partitioning tissue sections into an array of microwells containing barcoded primers for reverse transcription primers [35]. The High-Definition Spatial Transcriptomics (HDST) method uses microwell-based fluorescence spatial indexing beads to capture ...
The list of primers can be found in Supplementary Table S1. Blood parameters Blood samples were collected in EDTA-coated tubes and plasma was stored at −80 °C. Plasma levels of glucose, AST, ALT, total cholesterol, free fatty acids and triglycerides were assessed using a Cobas c 111...
(9 upregulated and 9 downregulated) were selected from each comparison group, andGapdhwas used as the internal reference gene for RT‒qPCR validation (Primers are shown in Supplementary document5). According to the results (Supplementary document5), the transcriptional sequencing results in this ...