★★★3.5整体, 平均评级为 3.5 星,共 5 星。 ★★★5 星,共 5 星。 ·8年前 staining of human tissue IF staining of formalin fixed, paraffin embedded human tissue, perfect experiment, strong band and clean background. 是否有用? 1 条仅评分的评论...
Snippet: ystem (Leica Biosystem Nussloch GmbH) by using a Leica BOND Polymer Refine Detection kit for anti-CC10 (Clone E11, Santa Cruz Biotechnologies, Inc., Santa Cruz, CA, USA) and anti-MUC5AC (AM32160PU-N, ACRIS Antibodies GmbH, Herford, Germany) primary antibodies...
MUC5AC levels of cells cultured on low adhesionplates were 10-fold higher than those of cells cultured on adhesion plates.Cells cultured on bovine serum albumin (BSA) coated plates, which produce low adhesion conditions, also induced the up-regulation of MUC5AC. Mucin staining byPAS and MUC5...
Distinct staining in more than 10% of the gastric cells was recorded as positive immunoreactivity for the relevant mucin. Tumors containing epithelial cells expressing only gastric or intestinal-type mucins were classified as having a gastric or intestinal mucin phenotype, respectively. Those containing ...
(c–e) Muc5ac messenger RNA increased 383-fold in OVA-challenged WT mice ( þ / þ ), but it was undetectable in Muc5ac knockout ( À / À ) mice (c), resulting in a 67% reduction in mucous metaplasia detected by periodic acid fluorescent Schiff's staining (d and e). ...
Traditionally, histochemical staining for mucin is also used for visualization of mucin inclusions for AC diagnosis in morphologically unclear cases5. However, the number of routine ancillary stains should be kept to a minimum not to waste tumor material that may be needed for treatment predictive ...
Genes for gel forming mucins—Muc2, Muc5ac, Muc5b, and Muc6 (Chicken:Lang et al., 2006). Mucinglycoproteinscan be neutral or acidic sialiated or acidic sulfated (chicken:Biasato et al., 2019). Greater mucin staining is reported in both thevilliand crypts in the ileum thanduodenumwith ...
In the present study, we confirmed that the SMAC treatment ameliorated the bronchial mucus hypersecretion as indicated by the AB-PAS and IHC staining, and decreased the expressions of MUC5AC and MUC5B both in vitro and in vivo. EGFR is a member of the ErbB family of receptor tyrosine ...
Systemic inflammatory response induced by diarrhea in piglets.ARepresentative H&E staining of colon of piglets. Scale bar = 100 μm.BColonic crypt depth in the two groups. The levels of TNF-α inCserum andDcolon. The levels of IL-8 inEserum andFcolon. The levels of IL-6 inGserum and...
A mucin deficiency can lead to extensive fluorescein staining such as that seen in this dry eye patient. There are two primary types of mucins that are produced within the body: membrane-spanning and secreted. The membrane-spanning mucins are embedded in the lipid bilayer of the cells, while ...