黏蛋白5AC是一种凝胶形成性黏蛋白,由表面黏液细胞分泌。糖皮质激素对于黏蛋白5AC mRNA的表达是必需的,而高剂量的氢化可的松会抑制其表达。此外,哮喘液能刺激黏蛋白5AC的合成增加数倍。促炎细胞因子IL-6和TNF-α刺激黏蛋白5AC的分泌,从而有助于慢性炎症引起的黏蛋白上调。黏蛋白5AC的表达依赖于视黄酸(RA)或...
(c–e) Muc5ac messenger RNA increased 383-fold in OVA-challenged WT mice ( þ / þ ), but it was undetectable in Muc5ac knockout ( À / À ) mice (c), resulting in a 67% reduction in mucous metaplasia detected by periodic acid fluorescent Schiff's staining (d and e). ...
MUC5AC (Mucin 5AC) Mouse Monoclonal IgG Antibody. Immunohistochemistry (IHC) applications. Human specificity
Muc5ac loading amounts were verified using the PM7 mixture of antibodies (Table 1). A negative control, without primary antibody incubation, was included for detection of background staining of non-specific secondary antibody binding. (c) Quantification of dot blot relative intensity. The ...
Immunostaining for DPC4 (nuclear transcription factor inactivated in some pancreatic carcinomas) and MUC5AC (a gastric mucin gene) is frequently positive in primary mucinous ovarian tumors.152 Staining for estrogen and progesterone receptors is typically not helpful in distinguishing primary mucinous ...
5. Apply blocking serum: 10 min (buffer 1, Dako). 6. At this point do not rinse the slides; clear the buffer. 7. Incubate at room temperature for 1 hr with primary antibody (AB2). 8. Rinse in PBS: 2 × 2 min. 9. Link, biotinylated secondary antibody (AB2): 25 min. ...
Five-micrometer cross-sections were deparaffinized, rehydrated, and used for immunohistochemical staining using target-specific primary antibodies and visualization with a secondary streptavidin–horseradish peroxidase antibody and 3-amino-9-ethylcarbazole (AEC) substrate to detect the expression and ...
Besides its reactivity with L56/C, a serum recognizing the bacterially expressed MUC5AC gene product, rM was endowed with M1 immunoreactivity: (i) rM-expressing cells were stained specifically with anti-M1 serum and with the monoclonal antibody (MAb) 21M1, defining the M1-f epitope; (ii) ...
Traditionally, histochemical staining for mucin is also used for visualization of mucin inclusions for AC diagnosis in morphologically unclear cases5. However, the number of routine ancillary stains should be kept to a minimum not to waste tumor material that may be needed for treatment predictive ...
Mucin extractability from sections of mouse eyes was assessed by lectin-blot, using helix pomatia agglutinin (HPA), and mucin content within goblet cells was assessed by immunohistochemistry, using an antibody specific to the goblet cell mucin Muc5AC.Altered mucin packaging in the goblet cells of ...