★★★3.5整体, 平均评级为 3.5 星,共 5 星。 ★★★5 星,共 5 星。 ·8年前 staining of human tissue IF staining of formalin fixed, paraffin embedded human tissue, perfect experiment, strong band and clean background. 是否有用? 1 条仅评分的评论...
This antibody clone 1-13M1 cross reacts with Mucin of different animal species after periodate pretreatment. Immunoblotting: Strongly positive without mercaptoethanol pretreatment of mucin solution. This antibody clone 1-13M1 recognizes Mucin 5AC under non-reducing conditions. ELISA ...
Muc5ac loading amounts were verified using the PM7 mixture of antibodies (Table 1). A negative control, without primary antibody incubation, was included for detection of background staining of non-specific secondary antibody binding. (c) Quantification of dot blot relative intensity. The ...
After blocking with the goat serum, the lung tissues were incubated overnight with an anti-MUC5AC antibody. 3,3-diaminobenzidine (DAB, ZSGB-BIO) was used to make the immunostaining visible. These sections were photographed using the microscope at 400×. 2.7. Analysis of bronchoalveolar lavage ...
(c–e) Muc5ac messenger RNA increased 383-fold in OVA-challenged WT mice ( þ / þ ), but it was undetectable in Muc5ac knockout ( À / À ) mice (c), resulting in a 67% reduction in mucous metaplasia detected by periodic acid fluorescent Schiff's staining (d and e). ...
Five-micrometer cross-sections were deparaffinized, rehydrated, and used for immunohistochemical staining using target-specific primary antibodies and visualization with a secondary streptavidin–horseradish peroxidase antibody and 3-amino-9-ethylcarbazole (AEC) substrate to detect the expression and ...
·5年前 Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. This antibody is recommended for Western blot of mouse samples, and we would expect it to be working with your stated conditions. We are unable to help you troubleshoo...
Besides its reactivity with L56/C, a serum recognizing the bacterially expressed MUC5AC gene product, rM was endowed with M1 immunoreactivity: (i) rM-expressing cells were stained specifically with anti-M1 serum and with the monoclonal antibody (MAb) 21M1, defining the M1-f epitope; (ii) ...
Secondary antibody used was horse-peroxidase goat anti-rabbit antibody in 25 mL of 5% milk at a dilution of 1:5000. Blots were developed using the horseradish flouro-illuminescence detection protocol using SuperSignal West Dura Extended Duration Substrate (Pierce, Rockford, IL) for 5 min. The ...
we created a genetic fusion to a nanobody rather than an antibody. The cell-surface receptor HER2 was selected as the target antigen because it is upregulated in several carcinoma subtypes, including breast and ovarian cancer, and is bound by a well-validated nanobody, 5F7 (ref.37). We ...