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产品名称货号加入购物车 mTOR Antibody2972 mTOR (7C10) Rabbit mAb2983 Phospho-mTOR (Ser2448) Antibody2971 Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb5536 Phospho-mTOR (Ser2481) Antibody2974 Rabbit anti-mTOR Polyclonal Antibodyabs131824 Rabbit anti-mTOR Polyclonal Antibodyabs145961 Rabbit anti-...
Western blot analysis of extracts from 293 cells (starved for 30 hours), untreated or treated with 20% FBS for 30 minutes, using Phospho-mTOR (Ser2481) Antibody (upper) or control mTOR Antibody #2972 (lower). 1/1 Learn more about how we get our images To Purchase # 2974 Cat. # ...
In addition, control blots for the mTOR antibody (Cell Signalling Technology #2972) were also missing. These are now provided below, as Fig. 9, and show that the lower band is likely non-specific.doi:10.1038/s41556-019-0280-yPengda Liu...
lomitapide and anti-PD-1 antibody significantly inhibited tumor growth compared to anti-PD-1 antibody treatment alone, in both MC38 and B16-F10 tumor models (Fig.6a, band Supplementary Fig.11A, B). Administration of 20 mg/kg of lomitapide resulted in no apparent changes in the body ...
Rabbit mTOR antibody (2972) was from Cell Signaling Technology. Blots were developed with the enhanced chemiluminescence detection kit (Amersham Pharmacia Biotech, Milan, Italy). Immunoblotting for IDO was performed with rabbit polyclonal IDO-specific antibody on DCs after 18-h stimulation.19 The ...
To determine whether PDI is associated with other factors related to post-radiation autophagy, we conducted Co-IP with the PDI antibody and then performed liquid chromatography with tandem mass spectrometry (LC-MS/MS). The LC-MS/MS data identified a set of proteins that are commonly involved in...
antibody (MA1-21315) was from ThermoFisher Scientific. Antibodies for actin (sc-47778), Myc (sc-40), and GST (sc-138 horseradish perxidase) were obtained from Santa Cruz Biotechnology. FLAG antibody (F7425) was obtained from Sigma–Aldrich. HA-tagged monoclonal antibody (901513) was from ...
Supernatants were incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and unbound proteins were extensively washed away. The fusion proteins (FLAG-tagged raptor, FLAG-tagged mLST8 and Myc-tagged mTOR) were digested using PreScission protease overnight and the eluted proteins were further ...
Bound proteins were then eluted and the presence of mTOR, PI3K alpha (PI3Kα), PI3K beta (PI3Kb), PI3K gamma (PI3Kg), PI3K delta (PI3Kd) and DNA-dependent protein kinase (DNA-PK) was detected and quantified using a specific antibody in a dot blot procedure and the Odyssey infrared ...