myostatin (MSTN) geneRNA interference为了筛选小鼠肌肉生长抑制素(MSTN)基因的干涉序列,根据GenBank中小鼠的MSTN基因序列,采用RT-PCR方法克隆获得MSTN基因序列,构建其真核表达载体pcDNA3.1(+)-MSTN;根据MSTN基因序列设计合成3种MSTN基因干涉序列(M1、M2、M3),并构建相应的RNA干涉载体pRNAT-M1、pRNAT-M2、pRNAT-M3。
Organization of the human myostatin gene and expression in healthy men and HIV—infected men with muscle wasting. Proc Natl Acad Sci USA, 1998, 95(25): 14938-14941 [5] 欧阳红生, 孙燕. 猪肌生成抑制素基因的克隆和序列测定[J]. 中国兽医学报, 2001, 21(5): 479-481 [6] Alexandra C Mcp...
1) Myostatin(MSTN) gene 肌生成抑制(MSTN)基因 2) Muscles/physiology 肌/生理学 3) pectoralis major growth 胸肌生长 4) myocardium/physiology 心肌/生理学 5) diaphragm/physiology 膈肌/生理学 6) Jisheng injection 肌生注射液 1. Objective: To observe the effect ofJisheng injection(JSI) in protecting...
Title: mstn Gene Mechanism 英文: The mstn gene, also known as muscleblind (MBNL) gene, plays a crucial role in muscle development and function.Mutations in the mstn gene can lead to muscle wasting and weakness, a condition known as muscleblindness or nemaline myopathy.The mstn gene encodes ...
1) MSTN gene MSTN基因1. And PCR amplification and sequencing was made on MSTN gene and the phylogenetic tree was constructed to discuss the phylogenetic relationship of MSTN gene exon 2 in 4 bovine species. [方法]采用酚-氯仿抽提法从4个中国牛种中提取基因组DNA,对MSTN基因进行PCR扩增和测序,...
cloningandbioformaticsanalysisonmyostatin40mstn41geneofgoat 系统标签: mstnbioformatics信息学myostatin山羊克隆 山羊肌生成抑制素(MSTN)基因的克隆 及生物信息学分析金鑫燕青海省畜牧兽医科学院,青海西宁810016 摘要:试验对山羊肌生成抑制素 myostatin,MSTN 基因序列进行了克隆及生物信息学分析,旨在为该基因的深入研究提...
The method comprises the following steps: (1) designing a specific nucleic acid primer aiming at a 5'UTR region of a goat MSTN gene and carrying out PCR amplification on a to-be-detected goat genome DNA to obtain 593bp of amplification product; (2) carrying out enzyme digestion and ...
关键词:动物遗传育种;牛;MSTN基因;作用机制;功能研究中图分类号:S813.3TheResearchProgressofMSTNGeneonBovineMuscleProliferationandtheRegulationofFatDepositionGaoFeng,XingShenyang,LiAonan,YuHaibin,ZhaoZhihui,YangRunjun(CollegeofAnimalScience,JilinUniversity,JilinChangchun,130062,China)Abstract:Myostatingene(Myostatin,...
This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. [provided by RefSeq, Jul 2008] [中文简述(自动翻译):] 由该基因编码的蛋白是骨形态发生蛋白(BMP)家族和TGF-β超家族的一个成员。该组蛋白的特征在于它被裂解以产生含有7个保守的半胱氨酸残基的成熟蛋白...
Whereas the negative muscle regulator myostatin (MSTN) in mammals is almost exclusively expressed in the muscle by a single encoding gene, teleost fish possess at least two MSTN genes which are differentially expressed in both muscular and non-muscular tissues. Duplicated MSTN-1 genes have ...