myostatin (MSTN) geneRNA interference为了筛选小鼠肌肉生长抑制素(MSTN)基因的干涉序列,根据GenBank中小鼠的MSTN基因序列,采用RT-PCR方法克隆获得MSTN基因序列,构建其真核表达载体pcDNA3.1(+)-MSTN;根据MSTN基因序列设计合成3种MSTN基因干涉序列(M1、M2、M3),并构建相应的RNA干涉载体pRNAT-M1、pRNAT-M2、pRNAT-M3。
Organization of the human myostatin gene and expression in healthy men and HIV—infected men with muscle wasting. Proc Natl Acad Sci USA, 1998, 95(25): 14938-14941 [5] 欧阳红生, 孙燕. 猪肌生成抑制素基因的克隆和序列测定[J]. 中国兽医学报, 2001, 21(5): 479-481 [6] Alexandra C Mcp...
1. Relationship between Polymorphism of Goat MSTN Gene Intron 2 and Body Weight MSTN基因内含子2多态性与山羊体重性状相关研究 www.ilib.cn 2. Study on MSTN Gene Expression in Big Bone Chicken 大骨鸡MSTN基因的表达检测 www.ilib.cn 3. Expression Rule of MSTN Gene in Big Bone Chicken ...
Title: mstn Gene Mechanism 英文: The mstn gene, also known as muscleblind (MBNL) gene, plays a crucial role in muscle development and function.Mutations in the mstn gene can lead to muscle wasting and weakness, a condition known as muscleblindness or nemaline myopathy.The mstn gene encodes ...
cloningandbioformaticsanalysisonmyostatin40mstn41geneofgoat 系统标签: mstnbioformatics信息学myostatin山羊克隆 山羊肌生成抑制素(MSTN)基因的克隆 及生物信息学分析金鑫燕青海省畜牧兽医科学院,青海西宁810016 摘要:试验对山羊肌生成抑制素 myostatin,MSTN 基因序列进行了克隆及生物信息学分析,旨在为该基因的深入研究提...
关键词:动物遗传育种;牛;MSTN基因;作用机制;功能研究中图分类号:S813.3TheResearchProgressofMSTNGeneonBovineMuscleProliferationandtheRegulationofFatDepositionGaoFeng,XingShenyang,LiAonan,YuHaibin,ZhaoZhihui,YangRunjun(CollegeofAnimalScience,JilinUniversity,JilinChangchun,130062,China)Abstract:Myostatingene(Myostatin,...
expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other...
The method comprises the following steps: (1) designing a specific nucleic acid primer aiming at a 5'UTR region of a goat MSTN gene and carrying out PCR amplification on a to-be-detected goat genome DNA to obtain 593bp of amplification product; (2) carrying out enzyme digestion and ...
关键词:IGF—I;MSTN基因;作用机理;研究进展中图分类号G804.2文献标识码A文章编号1671—5950(2009)03—0043—03ProgressesonStudiesofInsulin—likeGrowthFactorIandMyostatinGeneintheProcessofExercise—InducedCardiacHypertrophy//XUEFan,SUNBiaoAbstract:Thecardiachypertrophyinducedbyexerciseisanordina—ryphysicalphenomenonin...
Whereas the negative muscle regulator myostatin (MSTN) in mammals is almost exclusively expressed in the muscle by a single encoding gene, teleost fish possess at least two MSTN genes which are differentially expressed in both muscular and non-muscular tissues. Duplicated MSTN-1 genes have ...