By mediating the porcine MSTN gene by the zinc finger nuclease technique, base deletion is enabled, frameshift mutation is resulted in, early termination of translation is caused, an MSTN functional protein is unable to be formed, and the MSTN gene mutation sequence with 3782th to 3796th ...
inhibits growth of skeletal muscle and is considered as an important gene to improve lean meat production in stockbreeding.Studies have shown that knockouts of MSTN gene helps improve muscle development in animals.This paper aims to discuss the safety of MSTN gene knockout pigs obtained by gene ...
Abstract: Myostatin (MSTN) is one of the factors that regulate skeletal muscle development. It is a member of the TGF-βsuperfamily and can negatively regulate muscle growth. The gene is widely expressed in skeletal muscle, and deletion or mutation can cause animals to exhibit a 'bicuscular' ...
关键词 : MSTN 基因 ;外显子 ;遗传多态性 ;西门塔尔牛 DOI :10.13560/j.cnki.biotech.bull.1985.2015.06.026 Polymorphism Analysis of MSTN Gene Exons of Ganzhou Simmental Cattle in Gansu Province Li Jiyou1,4 Liang Chunhua2 Liu Xia1,3 Sun Xuejing2 Du Xiaohua1,2 (1. Gansu Key ...
whichwerecontrolledbytwoalleles.TheamplifiedfragmentsofAAandBBofthefragmentweresequenced.Theresultshowedthatin676bpBBgenotypetherewasonesubstitutionmutationatthe3783site(TC).Theresultabove—mentionedfirstcon-firmedthattherewerepolymorphismsinintron2(676bp)regionofMSTNgene.Keywords:goat;MSTN;geneticpolymorphism;RFLP;...
2) MSTN propeptide gene MSTN前肽基因 1. This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene,and verify its expression efficacy in C2C12 cells. 本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因,构建真核定点诱变载体,并...
A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. PLoS Genet 2007, 3, e79. [Google Scholar] [CrossRef] Schuelke, M.; Wagner, K.R.; Stolz, L.E.; Hubner, C.; Riebel, T.; Komen, W.; Braun, T.; Tobin, J.F.; Lee, S.J...
the mutation effi ci ency of gRNA2 is higher than gRNA 1 through the survery 万方数据 cRJSP刚 cRJSP刚caS9系统介导羊MSTN基因敲除和定点整合fat一1基因的研究 张驹 enzyme mutation detection,SO gRNA2 is used as follow-up experiments; Sequencing confirmed targeting gene fragment was obtained and the...
In contrast, a frame-shift mutation in exon 1 of salmon MSTN-2b would lead to the synthesis of a putatively non-functional truncated protein. The absence of processed MSTN-2b mRNA in the examined tissues indicated that this gene has become a non-functional pseudogene. The differential, but ...
关键词:MSTN基因;外显子;遗传多态性;西门塔尔牛DOI:10.13560/jki.biotech.bull.1985.2015.06.026PolymorphismAnalysisofMSTNGeneExonsofGanzhouSimmentalCattleinGansuProvinceLiJiyou1,4LiangChunhua2LiuXia1,3SunXuejing2DuXiaohua1,2(1.GansuKeyLaboratoryofHerbivorousAnimalBiotechnology,Lanzhou730070;2.CollegeofVeterinary...