The present invention discloses a method of introducing a frameshift mutation in the gene of bovine MSTN method. 本发明构建了一个牛MSTN基因移码突变的打靶载体,通过同源重组将移码突变引入牛MSTN基因第3外显子. The present invention constructed a bovine MSTN gene frameshift mutations targeting vector by ...
(98):9306-93ll 【5lSzaboG,DallmannG,MullerGeta1.A deletioninthemyostafingenecausesthe compact(Cmpt)hypermuscularmutationin mice.MalnnlGenome,1998(9):671-672 【6I 黎真,牛冬,阮晖,等.猪,鸡,鸭Myostatin 成熟区段DNA 的克隆及其同源性分析.畜 牧兽医,2005,36(4):323-327 【7IMcPherronAC,...
Abstract: Myostatin (MSTN) is one of the factors that regulate skeletal muscle development. It is a member of the TGF-βsuperfamily and can negatively regulate muscle growth. The gene is widely expressed in skeletal muscle, and deletion or mutation can cause animals to exhibit a 'bicuscular' ...
inhibits growth of skeletal muscle and is considered as an important gene to improve lean meat production in stockbreeding.Studies have shown that knockouts of MSTN gene helps improve muscle development in animals.This paper aims to discuss the safety of MSTN gene knockout pigs obtained by gene ...
In contrast, a frame-shift mutation in exon 1 of salmon MSTN-2b would lead to the synthesis of a putatively non-functional truncated protein. The absence of processed MSTN-2b mRNA in the examined tissues indicated that this gene has become a non-functional pseudogene. The differential, but ...
Molecular Cloning and Expression Analysis of the Myostatin Gene in Sea Perch ( Lateolabrax japonicus ) Myostatin (MSTN) is a member of the transforming growth factor-β (TGF-β) superfamily that functions as a negative regulator of skeletal muscle developmen... Han-Qing,Ye,Song-Lin,... - 《...
关键词:MSTN基因;外显子;遗传多态性;西门塔尔牛DOI:10.13560/jki.biotech.bull.1985.2015.06.026PolymorphismAnalysisofMSTNGeneExonsofGanzhouSimmentalCattleinGansuProvinceLiJiyou1,4LiangChunhua2LiuXia1,3SunXuejing2DuXiaohua1,2(1.GansuKeyLaboratoryofHerbivorousAnimalBiotechnology,Lanzhou730070;2.CollegeofVeterinary...
In this study we demonstrated the site-specific and mutation-specific modification at the pig MSTN gene to generate the isozygous, marker-free MSTN KO cloned pigs. This was achieved by the combined use of CRISPR/Cas9-mediated HR to knock out exon 3 of MSTN and Cre/LoxP recombination to ...
≮1¥摘要摘要本研究采用PCR—RFLP技术研究了我国主要的地方山羊品种以及引自国外的原产于欧洲及非洲的一些山羊品种及波尔山羊与唐山奶山羊杂交后代GHR,IGF.1,MSTN与MClR基因的多态性,结果如下:l用限制性内切酶Alul研究了14个山羊群体共140个个体GHK基因的ILFLP,结果发现该基因表现出单态。2用限制性内切酶HphI与B...
By mediating the porcine MSTN gene by the zinc finger nuclease technique, base deletion is enabled, frameshift mutation is resulted in, early termination of translation is caused, an MSTN functional protein is unable to be formed, and the MSTN gene mutation sequence with 3782th to 3796th ...