(MSP)检测组织DNA中OPCML基因甲基化发生率.结果 DNA序列中存在6个Bshl2361酶切位点,酶切24 h能完全消化非甲基化DNA,MSRE-PCR和巢式MSP检测卵巢癌组织中OPCML基因甲基化发生率分别为86.7%和84.4 %,正常卵巢组织两法均阴性.结论 MSRE-PCR检测卵巢癌组织OPCML基因甲基化发生率与巢式MSP相比没有显著性差异,是一种...
在此,研究人员报告了一种用于检测月经血和阴道液的四重甲基化敏感限制性内切酶 - 聚合酶链反应(4-plex MSRE-PCR)系统。该检测方法在多重聚合酶链反应(PCR)中使用了四种标记物:阳性对照(PC)、消化对照(DC)、月经血特异性(MB)标记物和阴道液特异性(VF)标记物。正如预期的那样,月经血和阴道液样本可通过 MB 和...
(MSP)检测组织DNA中OPCML基因甲基化发生率.结果 DNA序列中存在6个Bsh1236I酶切位点,酶切24 h能完全消化非甲基化DNA,MSRE-PCR和巢式MSP检测卵巢癌组织中OPCML基因甲基化发生率分别为86.7%和84.4%,正常卵巢组织两法均阴性.结论 MSRE-PCR检测卵巢癌组织OPCML基因甲基化发生率与巢式MSP相比没有显著性差异,是一种...
To address this limitation, we report on a novel 3-plex MSRE-PCR (methylation-sensitive restriction enzyme-PCR) assay to specifically identify spermatozoa. This assay is comprised of 3 markers: a digestive control marker (DC), sperm-specific marker (SP), and Y chromosome marker (SRY). A ...
The results also indicated that the 3-plex MSRE-PCR assay was readily accessible to forensic laboratories for integrating it into current forensic casework processes.Liu, Kuo-LanForensic Examination Division, Criminal Investigation Bureau, National Police Agency, Taipei, Republic of ChinaTsai, Li-Chin...
Validation of differential methylated regions mediated by PARylation using MSRE-PCR results on selected genes.Narasimharao NalabothulaTaha AljumailyAbdallah M. EteleebRobert M. FlightShao XiaorongHunter MoseleyEric C. RouchkaYvonne N. FondufeMittendorf...
MSRE-PCR for analysis of gene-specific DNA methylation. Nucleic acids research 2005; 33(10): e93.MELNIKOV A A;GARTENHAUS R B;LEVENSON A S;.MSRE-PCR for analysis of gene-specific DNA methylation.Nucleic Acids Rec.2005.3011-3019Melnikov AA, Gartenhaus RB, Levenson AS, Motchoulskaia NA, Lev...
1143P Virtual amplicons for methylation-sensitive restriction enzyme quantitative PCR (MSRE-qPCR) derived from genome-wide DNA methylation sequencing: Application to prediction of breast cancer neoadjuvant chemotherapy responsedoi:10.1016/j.annonc.2021.08.784A.I. Kalinkin...