本研究运用自行建立的甲基化敏感性限制性内切酶一定量PCR(methylation-sensitive restriction enzymes-based quantitatire PCR,MSRE-qPCR)方法检测肝细胞癌(hepatocellutar carcinoma,HCC)组织中多个基因的DNA甲基化状态,筛选可用于HCC诊断的DNA甲基化标志物组合。方法:以47对HCC患者癌组织和癌旁组织、8例正常人肝组织为...
在此,研究人员报告了一种用于检测月经血和阴道液的四重甲基化敏感限制性内切酶 - 聚合酶链反应(4-plex MSRE-PCR)系统。该检测方法在多重聚合酶链反应(PCR)中使用了四种标记物:阳性对照(PC)、消化对照(DC)、月经血特异性(MB)标记物和阴道液特异性(VF)标记物。正如预期的那样,月经血和阴道液样本可通过 MB 和...
目的 建立精子中父源印记基因H19上游区域MSRE-qPCR检测方法,并分析男性少弱精子症患者精子父源印记基因H19上游区域甲基化水平.方法 收集20例正常精液样本,同时筛选30例少弱精子症患者精液样本,应用甲基化敏感性限制性内切酶法并结合定量PCR对所有样本父源印记基因H19上游区域 甲基化水平进行分析.结果 正常精液样本父源...
MSREs cleave DNA at specific unmethylated-cytosine residues and DNA is then amplified by PCR following digestion. Because unmethylated DNA is digested by MSREs, the only amplification products detected are methylated DNA. It is important to fully digest the DNA before PCR amplification to generate ...
GENT-42. SIMULTANEOUS MEASUREMENT OF MGMT METHYLATION AND DETERMINATION OF IDH1 R132H AND CpG ISLAND METHYLATOR PHENOTYPE (CIMP) IN GLIOMAS USING COUPLED ABSCRIPTION PCR SIGNALING (CAPS) Tumor samples received by clinical laboratories often contain insufficient amplifiable DNA to measure gene-specific ...
卵巢癌目的 建立甲基化敏感性限制性内切酶-PCR(MSRE-PCR)法检All OPCML基因,探讨其在卵巢癌中的意义.方法 用亚硫酸氛钠修饰测序法检测5例卵巢癌组织及正常卵巢组织中OPCML基因甲基化,分别用MSRE-PCR和巢式甲基化特异性PCR(MSP)检测组织DNA中OPCML基因甲基化发生率.结果 DNA序列中存在6个Bshl2361酶切位点,酶切24...
(MSP)检测组织DNA中OPCML基因甲基化发生率.结果 DNA序列中存在6个Bsh1236I酶切位点,酶切24 h能完全消化非甲基化DNA,MSRE-PCR和巢式MSP检测卵巢癌组织中OPCML基因甲基化发生率分别为86.7%和84.4%,正常卵巢组织两法均阴性.结论 MSRE-PCR检测卵巢癌组织OPCML基因甲基化发生率与巢式MSP相比没有显著性差异,是一种...
Mixture analyses illustrated the sensitivity of the assay when the vaginal/semen DNA ratio (80/0.1) was under 800 or the menstrual blood/semen DNA ratio (5/0.1) was under 50, the trace amounts (approximately 0.1ng) of DNA from semen can still be identified by this 3-plex MSRE-PCR ...
MSRE-PCR for a-nalysis of gene-specific DNA m ethylation. M eln ikov AA,Gartenhaus RB,Levenson AS,et al. Nucleic Acids Research . 2005M eln ikov AA,Gartenhaus RB,Levenson AS,et al.MSRE-PCR for a-nalysis of gene-specific DNA m ethylation.Nucleic Acids Research. 2005...
Validation of differential methylated regions mediated by PARylation using MSRE-PCR results on selected genes.Narasimharao NalabothulaTaha AljumailyAbdallah M. EteleebRobert M. FlightShao XiaorongHunter MoseleyEric C. RouchkaYvonne N. FondufeMittendorf...