R92TC-2) was added to each well and plate read on an MSD plate reader (Meso-Scale Diagnostics, Sector S600).For ACC MSD assays, MSD Streptavidin gold plates (Meso-Scale Devices, L15SA-2) were blocked in 1% Blocker A (Meso-Scale Diagnostics, R3AA-2)...
h. Wells were washed three times with 1×Tris Wash Buffer, 150 μL of 1× MSD Read Buffer (Meso-Scale Diagnostics, R92TC-2) was added to each well and plate read on an MSD plate reader (Meso-Scale Diagnostics, Sector S600). For ACC MSD assays, MSD Streptavidin gold plates (Meso-S...
Safety and Regulatory Information 8 3 General Operation and Hazards 3.1 General Operation The instrument's camera must cool to the appropriate temperature before reading an assay plate. The instrument must be connected to the computer via the MSD-provided USB cable. Power on the instrument and ...
引用MSD摘要:FACS-purified neurons were plated at 2 3 105 per well of a 96-well plate. Cells were cultured for an additional 5 days with a full media change on day 3. Amyloid-b was measured with MSD Human (6E10)...
antibody was added to each well after shaking the whole plate on an orbital shaker at the speed of 700 rpm/min overnight at 4◦C. Each well was washed three times with 0.05% PBST before adding 2X reading buffe...
(MSD), Rockville, MD). A MULTI-SPOT® 96-well Plate was coated with SARS-CoV2 antigensSpike, and RBDat 200 μg/mL in PBS, washed, dried and packaged for further use (MSD® SARS-CoV-2 Plate 2). Internal quality controls and reference standard reagents were developed from pooled ...
• The front side plate screw should not be tightened. • The MSD is connected to a grounded power source. • The GC/MSD interface extends into the GC oven. • A conditioned capillary column is installed in the GC inlet and in the GC/MSD interface. • The GC is on, but the...
read on an MSD plate reader (Meso-Scale Diagnostics, Sector S600). 和普通WB相比,MSD电化学发光检测的优势: 1、实验流程更为简便快捷:MSD仅需4.5小时就可以完成整个实验;而传统的WB则需要16小时。 2、灵敏度更高,可发现原来发现不了的差异,见下图(即使0.3ug的上样量仍能检出磷酸化差异,说明极小磷酸化...
antibody was added to each well after shaking the whole plate on an orbital shaker at the speed of 700 rpm/min overnight at 4◦C. Each well was washed three times with 0.05% PBST before adding 2X reading buffer. Electrochemiluminescence was measured using a MESO QuickPlex SQ 120 reader....
(MSD), Rockville, MD). A MULTI-SPOT® 96-well Plate was coated with SARS-CoV2 antigensSpike, and RBDat 200 μg/mL in PBS, washed, dried and packaged for further use (MSD® SARS-CoV-2 Plate 2). Internal quality controls and reference standard reagents were developed from pooled ...