Luminescence intensity was quantified using a Multimode Plate Reader (PerkinElmer) luminescence counter. Data were normalized to GoA reference and analyzed using GraphPad Prism 9.2.0. Radio-ligand binding assay Purified M2R receptor was reconstituted into high-density lipoprotein (HDL) particles ...
Absorbance was then measured at 490 nm using an EnSpire multimode plate reader (Perkin Elmer). To convert the absorbance value to the amount of melanin, a standard curve was obtained from 0 to 500 μg/mL of synthetic melanin (Sigma-Aldrich) solution dissolved in 1 N NH4OH. When ...
The non-infected cell control (mock) and the untreated HIV-1 infected cells (Virus control or VC) were included in the anti-HIV-1 assays. The preinstalled software of the Perkin Elmer multimode Luminometer system calculated the percentage inhibition of HIV-1 infection of the extracts along with...
G Protein-Coupled Receptor Assays are Highly Comparable on the PerkinElmer® EnSpire™ Multimode Plate Reader with Epic® Label-Free Technology and t... each agonist on the two instruments were compared. Kinetic monitoring of the dynamic mass redistribution (DMR) that occurs in living cells ...
Increasing fluorescence intensity was recorded as the reaction progressed (excitation wavelength: 300 nm, emission wavelength: 410 nm) on an Enspire multimode plate reader, PerkinElmer. All reactions were performed at 37°C in 50 mM Tris-HCl, pH 7.5, 10 mM NaCl. Calibration curves was created ...
Another sub-sample of the same biofilm slurry was measured for enzyme activities using a 96-well microplate spectral scanning multimode reader (fluorescence intensity mode with ex: 365 nm, em: 450 nm) (Varioskan Flash; Thermo Fisher Scientific, USA). 10 mL of the original samples were diluted...
The quantity and quality of the DNA samples were assessed using Nanodrop ND-1000 Spectrophotometer (Thermo Scientific, USA) and Quant-i Picogreen assay (Invitrogen, USA) on Varioskan Flash Multimode Reader (Thermo Scientific, USA). The DNA samples were diluted to a final concentration of 20-50...
Multimode (MMI) Volatiles interface (VI) Transfer line through GC inlet top Standard configuration Cool on-column (CoC) Purged packed (PP) Transfer line through GC inlet septum Optional configuration S/SL or MMI with 7890A transfer line interface ...
After treatment both cells were lysed and measured luciferase activity using a Promega Luciferase Assay system (Promega Corporation) by a Varioskan flash Multimode Microplate Reader (Thermo Scientific). RT–qPCR and Western Blotting The mRNA expression level of S3-15 was measured by RT–qPCR as ...
An EnSpire Multimode Microplate Reader (PerkinElmer, Waltham, MA, USA) was used to detect the fluorescence intensity in the MAX solution. The secondary antibodies conjugated to different fluorophores were diluted 10 times with PBS, iMAX, and sMAX in a 96-well black microplate. After incubation at...