Antigen: MS2 Coat Protein Accession ID: P03612 Molecular Weight: 13 kDa Clonality: Monoclonal Clone Name: 3H4 Immunogen: Full length recombinant MS2 coat protein Species Immunized: Mouse Purification Method: Not purified Buffer: Ascites Fluid Tested Applications: WB (1:2500) Storage...
L.Purification and properties 效序列为47YGRKKRRQRRR57。将内含反义RNA的 of bacteriophage MS2 and of its ribonucleic acid[J].J Mol Bi— MS2 VLP与HIV一1 Tat ,转导肽通过化学交联剂 ol,1963,7:43-54. Sulfo～SMPB交联,在Tat。,。转导肽的作用下将VLP ...
By using the specific interaction between MS2 coat protein and MS2 RNA hairpin, we developed a method (MS2-tagged RNA affinity purification and mass spectrometry (MTRAP-MS)) to identify proteins that interact with MEG3. Mass spectrometry and gene ontology (GO) analysis showed that MEG3 binding...
BL21 (DE3) bacterial lysate showed both monomers and dimers of coat protein (Supplementary Fig. S1). The influence of different NaNO3 concentrations (1 mM and 100 mM, pH:8) and pH values (7 and 8) were assessed in MS2 VLP expression, production, and purification by the PEG precipitation ...
Subsequently, vector DNA was purified using the QIAquick PCR purification kit (Qiagen). Ligation of the fragment encoding the maturase and single-chain version of the coat protein dimer with the His-tag modification to the MCS1 of the pACYCDuet-1-TM vector was performed using the Quick-...
Cellular proliferation increased between days 3–6 post stimulation, suggesting that this response was specific for wt MS2 coat protein and not due to any residual E. coli-derived mitogenic contamination following protein purification. wt MS2 elicited a significant but much reduced proliferation response...
Coat ProteinMS2 OperatorQβ OperatorStructural Interpretation MS2 wild-type 2.4 50 Qβ RNA A+7 causes a steric clash with Asn87. Qβ wild-type 100 3.6 MS2 87Ser 25 20 Creates more room to allow the Qβ RNA A+7 stacking interaction MS2 87Ser, 89Lys 25 5.3 The improved affinity for...
A chimaeric protein, suitable for incorporation in a vaccine and capable of forming parts of a capsid assembly, comprises the amino acid sequence of the coat protein of phage MS-2, or a conservatively modified variant thereof, or sufficient of said sequence or variant to retain capsid-forming ...
The approach combines gel filtration and an affinity-chromatography strategy using the bacteriophage MS2 coat protein, which binds to a specific RNA-hairpin structure. Using this method, highly purified and functional human spliceosomes have been isolated. The purified spliceosome preparation is used to...
COAT proteins (Viruses)VIRUS removal (Water purification)[Objective] Using the characteristics of MS2 phage coat protein that can self-assemble and encapsulate exogenous nucleic acid in vitro, prepare RT-PCR quality control materials for detection of porcine epidemi...