Product Type:Antibody Antigen:MS2 Coat Protein Accession ID:P03612 Molecular Weight:13 kDa Clonality:Monoclonal Clone Name:3H4 Immunogen:Full length recombinant MS2 coat protein Species Immunized:Mouse Purification Method:Not purified Buffer:Ascites Fluid ...
MS2 Coat Protein polyclonal antibody (Merck Millipore, USA) and secondary goat anti-rabbit IgG conjugated with HRP (Jackson ImmunoResearch, UK) antibodies at wild type MS2 bacteriophage coat protein (B1) and single chain version of the MS2 coat protein dimer containing the His-tag (B2); (C) ...
To confirm the expression of the coat protein of VLPs, ~ 14 kDa disassembled protein of purified MS2 VLPs was separated by electrophoresis on 12.5% acrylamide gel and detected using specific antibody and Western blot method. BL21 (DE3) bacterial lysate showed both monomers and dimers of coat...
The experiments described here are aimed at satisfying the first of these two requirements by engineering efficient peptide display at two different sites in MS2 coat protein. First, we evaluated the suitability of the N-terminus of MS2 coat for peptide insertions. It was observed that random N-...
The experiments described here are aimed at satisfying the first of these two requirements by engineering efficient peptide display at two different sites in MS2 coat protein. First, we evaluated the suitability of the N-terminus of MS2 coat for peptide insertions. It was observed that random N-...
Previously we used bacteriophage MS2 coat protein fusions to tether either hnRNP A1 [6] or TIA-1 [7] to a pre-mRNAmoleculecontaininganRNAhairpinthatbinds MS2coat.Tetheringbothproteinstothesamepre-mRNA moleculeimpliesusingtwofusionpartnersofdi?erentRNA Alternativesplicingcontrolisvitalforcorrectgeneexpres-...
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Antibody titers were determined by considering the reciprocal of the highest sera dilutions at which the reactivity of experimental sera was greater than 2-fold compared to control sera at the same dilution. 2.4. Genital Infection HPV pseudoviruses (PsVs) representing HPVs 5, 6, and 51 were ...