Full size image Recently, we published a comprehensive review of mRNA-based vaccines and therapeutics [2], providing an overview of the structural elements and chemical modifications of mRNA, various delivery systems, administration routes, and potential clinical applications of these therapeutics. The p...
Previous research has highlighted that system viscosity and surface tension play crucial roles in determining the properties of aerosols generated through nebulization, including factors like fine particle fraction (FPF) and total output36,37. Specifically, rising viscosity correlates with an increase in F...
Regarding mRNA biomolecules, exogenous RNA can expose individuals to endothelial damage, intercellular junction relaxation, edema, increased viscosity, hypercoagulation, and thromboembolic events due to the immunogenicity of the exported RNA19. To abate exogenous RNA-induced immunogenicity, two technical appro...
The average particle size of the polyplexes was determined twice by dynamic light scattering using the Zetasizer 3000 (Malvern Instruments, Malvern, UK) with the following specifications: automatic sampling time, 10 measurements per sample, medium viscosity 0.89 cP, refractive index medium 1.33, ...
New low-viscosity overlay medium for viral plaque assays. Virol. J. 2006; 3: 63. Google Scholar Crossref PubMed WorldCat 50. Hallegger M., Chakrabarti A.M., Lee F.C.Y., Lee B.L., Amalietti A.G., Odeh H.M., Copley K.E., Rubien J.D., Portz B., Kuret...
Al-Kuraishy HM, Al-Gareeb AI, El-Bouseary MM, Sonbol FI, Batiha GE. Hyperviscosity syndrome in COVID-19 and related vaccines: exploring of uncertainties. Clin Exp Med. 2022;1-10. Published online May 24, 2022. doi:10.1007/s10238-022-00836-xPubMedGoogle ScholarCr...
viscosityThe mechanism of transport of mRNA-protein (mRNP) complexes from transcription sites to nuclear pores has been the subject of many studies. Using molecular beacons to track single mRNA molecules in living cells, we have characterized the diffusion of mRNP complexes in the nucleus. The ...
Viscosity reduction by DNA-shearing If the raw extract is noticeably viscous due to released DNA, a DNA-shear step should be included in your protocol. Use force to shear the DNA properly by passage through a syringe. In addition, be aware that repeat...
30, 31, 32 Moreover, sucrose increases the viscosity of the modRNA solution and prevents clumping of single-stranded modRNAs in the mixture, clumping that may result in untranslatable double-stranded modRNA that may also elicit an immune response via Toll-like receptor 3.33 Additionally, citrate...
Introduction Eukaryotic mRNA is canonically modified by the addition of the 5ʹ cap and bears additional modifications at internal sites. TheN6-methylation of adenosine (m6A) is the most abundant and best-studied internal modification of mRNA. It has been linked to cellular differentiation, cancer...