mRNA trans-splicing in the human parasitic cestode Echinococcus multilocularis. J. Biol. Chem. 275: 38311-38318.Brehm K,Jensen K,Frosch M. mRNA trans-splicing in the human parasitic cestode Echinococcus multilocularis.[J].The Journal of biological chemistry 2000,49(49)....
反式剪接(trans splicing):来源于不同前体mRNA中的外显子;经过剪切和拼接;形成一个成熟mRNA分子;分子间的特殊剪接方式。 题目要求解释“反式剪接”的定义及特点。根据描述,反式剪接的核心特征是不同前体mRNA的外显子拼接为同一成熟mRNA,属于分子间的剪接方式。题目给出的四个要点完整覆盖了定义(外显子来源)、过...
In addition, we provide evidence that alternative splicing of variable exons within a gene cluster occurs via a cis-splicing mechanism. However, virtually every variable exon can engage in trans-splicing with constant exons from another cluster, albeit at a far lower level....
The "spliced leader" type, found in protozoans such as trypanosomes and lower invertebrates such as nematodes, results in the addition of a short, capped 5' noncoding sequence to the mRNA. The "discontinuous group II intron" form of trans-splicing, found in plant/algal chloroplasts and ...
The protein coding sequences of most eukaryotic messenger RNA precursors (pre-mRNAs) are interrupted by non-coding sequences called introns. Pre-mRNA splicing is the process by which introns are removed and the protein coding elements assembled into mature mRNAs. Alternative pre-mRNA splicing selecti...
Article https://doi.org/10.1038/s41467-024-46172-4 Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing Received: 2 January 2024 Accepted: 16 February 2024 Check for updates David N. Fiflis 1, Nicolas A. Rey 2, Harshitha Venugopal-Lavanya 1, Beatrice Sewell2, Aaron Mitchell-...
We present a plasmid-based system in which upstream trans-splicing efficiently generates mRNAs that encode head-to-tail protein multimers. In this system, trans-splicing occurs between one of two downstream splice donors in the sequence encoding a C-term
Alternative pre-mRNA splicing is a critical mechanism that generates multiple mRNA from a single gene, thereby increasing the diversity of the proteome. Recent research has highlighted the significance of specific splicing isoforms in cellular processes,
关键词:SL反式剪接;剪接引导序列;剪接引导序列RNA 中图分类号:Q753文献标识码:A文章编号:1006—7167(2008)0l一0017—06 SplicedLeaderTrans—splicing:AConservedModification MechanismofPre.mRNA ZHENGYa—dong,LUOXue—nong,ZHANGDongng,CAIXue-peng (KeyLab.ofVeterinaryParasitologyofGansuProvince,StateKeyLab.of ...
trans-splicing; and (b) a second nucleic acid sequence comprising a second promoter operably linked to a nucleic acid sequence encoding a second trans-spliceable pre-mRNA sequence comprising at least one exon encoding a 3′ fragment of said transcript of interest and a second RNA sequence ...