Both categories of trans-splicing are mechanistically similar to conventional nuclear pre-mRNA cis-splicing; potential evolutionary relationships are discussed.doi:10.1096/fasebj.7.1.8422973Bonen LThe FASEB JournalBonen,L. (1993) Trans-splicing of pre-mRNA in plants, animals and protists. FASEB J....
真核生物中,只需要tRNA3'加工内切酶(tRNA 3’-processing endoribonuclease, 3’-tRNase)负责tRNA前体3'端加工。 4.3 反式剪接(Trans-splicing) 之前我们讨论的都是顺式剪接(cis-splicing),涉及的是存在于一个基因上的多个外显子。反式剪接指的是涉及不在同...
跨外显子复合体转变为跨内含子复合体,并跳过中间的外显子;3)若下游的U1 snRNP被结合则介导反向剪接,形成环状RNA(circRNA);4)若来自另一个转录本的U1 snRNP被结合,则介导反式剪接(trans-splicing)。
Pre-mRNA splicing A post-transcriptional modification of pre-mRNA, in which introns are excised and exons are joined in order to form a translationally functional, mature mRNA. γ-phosphate The third phosphate attached at the 5′ end of the ribose moiety of a nucleotide. 'Ping-pong' mechanism...
We present a plasmid-based system in which upstream trans-splicing efficiently generates mRNAs that encode head-to-tail protein multimers. In this system, trans-splicing occurs between one of two downstream splice donors in the sequence encoding a C-term
3. Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing 译：重新利用CRISPR-Cas13 系统实现稳健的 mRNA 转拼 作者列表:Fiflis DN;Rey NA;Venugopal-Lavanya H;Sewell B;Mitchell-Dick A;Clements KN;Milo S;Benkert AR;Rosales A;Fergione S;Asokan A 发表日期: 2024 Mar 14 期刊:Nat ...
5.1 Capping 真核生物转录产物：直接使用吗？Peptidechain 5‟ mRNA RNApolymeraseDirectionoftranscriptionRibosome3‟3‟-OH5‟ DNA5‟3‟ NucleusDNACapPre-mRNAIntron1Exon1Intron2Exon3 Exon2TranscriptionSplicing PolyadenylationMaturemRNANuclearexport-AAA---ACytoplasm MaturemRNATranslation -AAA---A...
以上所述为分子内剪接(cis-splicing),而mRNA剪接作用亦可发生在不同mRNA分子之间(trans-splicing),后者常见于原生生物的mRNA剪接[来源请求]。除了mRNA之外,有些RNA分子也有能力催化自身的修剪,例如核酸酶会做自我切除,而第I或第II型外显子在特殊环境下可以不借由蛋白质酵素的作用而进行剪接,称为自剪接作用。多聚...
Here, we present a dual AAV vector technology based on reconstitution via mRNA trans-splicing (REVeRT). REVeRT is flexible in split site selection and can efficiently reconstitute different split genes in numerous in vitro models, in human organoids, and in vivo. Furthermore, REVeRT can ...
Trans splicing of messenger RNA has been used in experimental settings to replace mutant RNA sequences. We investigated the feasibility of utilizing trans splicing to replace a mutant RET protooncogene sequence known to inappropriately activate this tyrosine kinase receptor. We constructed a pre-trans-...