Three amino acid transporter genes (AAP3-5) were isolated from Arabidopsis by complementation of a yeast mutant defective in histidine uptake. Transport is driven against a concentration gradient and sensitive to protonophores. Analysis of the substrate specificity demonstrates that the carriers have a...
mRNA疫苗设计和制备: A plasmid encoding the RNA polymerase promoter followed byhAg 5’UTR, with optimized Kozak sequence, and ORF,hAg 3’UTR, and120nt poly(A)tail were synthesized. The ORF coding for KRAS G12V neo-a...
(3)By virtue of its length or amino acidcomposition, it allows the clearest description ofdomains...
与单链mRNA 或 microRNA互不配对儿的“另外一条mRNA或 microRNA”）.cDNA is often used to clone ...
a ribosome to produce a specific amino acid chain or polypeptide. There is a balance between the processes of translation and mRNA decay24,25(Fig.4). It has previously been implicated that these structural elements that are being actively translated also intimately connect to mRNA decay, ...
mRNA vaccines represent a promising alternative to conventional vaccine approaches, but their application has been hampered by instability and delivery issues. Here, Pardi and colleagues discuss recent advances in mRNA vaccine technology, assess mRNA vac
在mRNA递送领域,多种含胺基的聚酯已被开发用于mRNA递送,包括聚(β-氨基酯)[poly(β-aminoester),PBAEs,4]、氨基聚酯(amino polyester,APEs)和电荷改变释放转运体(charge-altering releasable transporters,CARTs,5和6)等。其中,PBAEs 是经典...
A close examination of their codon pattern and analysis of RNA structure propensity led to the following set of empirical rules: (1) One class of amino acid repeats (Class I) uses a mixture of synonymous codons, some of which approximate the codon bias ratio in the overall human proteome; ...
Messenger RNA, molecule in cells that carries codes from the DNA in the nucleus to the sites of protein synthesis in the cytoplasm (the ribosomes). Each mRNA molecule encodes information for one protein. In the cytoplasm, mRNA molecules are translated fo
5. Brenner, S., F. Jacob, and M. Meselson, An unstable intermediate carrying information from genes to ribosomes for protein synthesis. Nature, 1961. 190: p. 576-581. 6. Gros, F., et al., Unstable ribonucleic acid revealed by pulse labelling of Escherichia coli. Nature, 1961. 190:...