CPC2: a fast and accurate coding potential calculator based on sequence intrinsic features. Nucleic Acids Res. 2017;45:W12–6. Article CAS PubMed PubMed Central Google Scholar Nagano T, Mitchell JA, Sanz LA, Pauler FM, Ferguson-Smith AC, Feil R, et al. The air noncoding RNA ...
(non-coding sequence). initially, it is produced from a dna sequence inside the nucleus by transcription. it undergoes many processing stages before being converted into mrna or mature messenger rna. the most important processing stage is rna splicing, where the introns are removed. the other ...
the NSPlDN1 strain was also generated by transforming only theniaDmarker into the NSPlD1 strain. The MBS-containing vector sequences were then amplified by inverse PCR using PrimeSTAR MAX DNA polymerase (Takara
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It was demonstrated that inverted complementary repeats within coding DNA sequences (ORFs) should be considered for proper estimation of the translation efficiency because they can form secondary structures that obstruct ribosome movement. A program was developed for estimating the potential expression of ...
Long-chain non-coding RNA (lncRNA) is closely related to many biological activities. Since its sequence structure is similar to that of messenger RNA (mRNA), it is difficult to distinguish between the two based only on sequence biometrics. Therefore, it
CPC: assess the protein-coding potential of transcripts using sequence features and support vector machine. Nucleic Acids Res. 2007;35(Web Server issue):W345–9. Article PubMed PubMed Central Google Scholar Huang DW, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene ...
Coding potential analysis and Target gene prediction. We used CNCI, CPC, PFAM and phyloCSF to distinguish mRNA from lncRNA. CNCI profiles can effectively distinguish protein-coding and non-coding sequences independent of known annotations by adjoining nucleotide triplets60. CPC searches the ...
oryzae genomic DNA sequence. We used pANPXP-Cre to insert a 36×MBS sequence downstream of the glaA gene by Cre/loxP system (Zhang et al., 2017), and 939 bp of the 3′ end of the glaA ORF and 950 bp downstream were amplified by PCR. First, a NotI restriction enzyme site ...