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During preparation, the supercoiled plasmid is cleaved using a restriction enzyme (BsaI) at the 3’ end of the poly(A) tail to generate a linear template that prevents readthrough transcription (Fig. 4e). To measure the efficiency of plasmid linearization, HPLC, or agarose gel electrophoresis i...
One of the regulatory elements that may be present in the 5 ′ UTR is the upstream Open Read-Journal of...Wilkie, G.S., Dickson, K.S., and Gray, N.K. (2003). Regulation of mRNA translation by 5′- and 3′-UTR-binding factors. Trends Biochem. Sci. 28, 182-188....
Read our blog NewsJob opportunities Moderna Announces Updates on Pandemic Influenza Program View all news 13+ Years of progress 45 Development programs 36 Ongoing clinical trials Meet Moderna Moderna's mission is to deliver the greatest possible impact to people through mRNA medicines. ...
(2021). From yeast to mammals, the nonsense-mediated mrna decay as a master regulator of long non-coding rnas functional trajectory. Non-coding RNA, 7(3), 44. Article CAS PubMed PubMed Central Google Scholar Maquat, L. E., Tarn, W. Y., & Isken, O. (2010). The pioneer round...
An important open question is whether elongation rate is regulated to effect changes in alternative mRNA processing. Slow elongation lengthens the 'window of opportunity' for an upstream event to occur before it faces competition from a downstream RNA sequence element; conversely, fast elongation '...
Endometriosis (EMs), the ectopic planting of functional endometrium outside of the uterus, is a leading cause of infertility and pelvic pain. As a fundamental mRNA modification, N6-methyladenosine (m6A) participates in various pathological processes. How
During viral infection there is dynamic interplay between the virus and the host to regulate gene expression. In many cases, the host induces the expression of antiviral genes to combat infection, while the virus uses “host shut-off” systems to better
However, their optimization is hindered by mRNA instability and inefficient protein expression. Here, we describe design principles that overcome these barriers. We develop an RNA sequencing-based platform called PERSIST-seq to systematically delineate in-cell mRNA stability, ribosome load, as well as ...
mRNA derived from autologous tumor cells or tumor stem cells contains all the proteins in tumor cells. This strategy is simple and feasible; however, its targeting and effectiveness need to be improved, and safety should also be considered. 2.1.2. Tumor-specific antigens TSAs are usually tumor...