Additional mutations in 5A had no effect on FoMV replication in protoplasts but rendered the virus noninfectious to plants. A correlation with diminished CP production from both mutant clones implies that synthesis of subgenomic CP mRNA was compromised, and this limited systemic infection. 展开 ...
As RBPs participate in posttranscriptional regulation usually via the 3′-UTR and 5′-UTR, we compared the sequence of MEKK1 mRNA 3′-UTR and 5′-UTR with the IMP3 potential binding site. Results showed that the MEKK1 3′-UTR contains multiple potential IMP3 binding sites, while there ...
2,3,4,5], first described as a regulator of transcription [6,7,8,9,10]. It has since been shown to have key regulatory roles extending well beyond transcription. It is a major complex involved in regulating an mRNA throughout the entire mRNA life cycle including facilitating mRNA export ...
88,89. We generated transgenic plants with two amiRs driven by constitutive promoters in one T-DNA which was named as osclsy3-kd2 (double amiR) (Supplementary Fig.6A, B). To silenceOsCLSY3in a dose-dependent manner, we also generated transgenics expressing single amiR (Supplementary Fig.6A...
They have been injected with two mRNAs encoding each monomer of a specific ZFN engineered by Sigma or with a tricistronic mRNA encoding EGFP-ZFN1-ZFN2 and engineered by ourselves. In the tenth experiment, 23 EGFP-positive embryos were transferred into eight recipient goats, from which five ...
There is clear evidence that the oceans are warming due to anthropogenic climate change, and the northeastern coast of USA contains some of the fastest war
Figure 3 Expression of a cone-specific marker and nestin in 661W cells. (A) Semi quantitative PCR analysis for mRNA expression of cone-specific marker OPN1MW and neuronal precursor marker nestin. (B,C) Western blot analysis of 661W and RGC-5 cells showing the expression of OPN1MW and nest...
13 Cells were treated with 100 μg/mL cycloheximide for 5 minutes at 37°C before collection. The cells were then lysed and treated with RNAse I to degrade unprotected mRNA regions. The intact mRNA-ribosome complexes were isolated and sent for sequencing with the Illumina HiSeq 4000 (SE50)...
This modulation is especially interesting considering the existence of postsynaptic pools of mRNA localized in the synaptic spine [17]. Previous work from our group has demonstrated that protein synthesis inhibition by cycloheximide (CHX) can stimulate L-citrulline production in neuronal cultures [16]....
The viral RNA 3′- and 5′-end structure and mRNA transcription of infectious salmon anaemia virus resemble those of influenza viruses. Arch Virol. 145, 1659–69 (2000). Article CAS PubMed Google Scholar Valenzuela-Miranda, D., Boltana, S., Cabrejos, M. E., Yanez. J. & Gallardo-...