Influenza A virus NS1 effector domain is required for PA-X-mediated host shutoff in infected cells. J Virol 98, e01901-23. 10.1128/jvi.01901-23 Lothstein, K.E., Chen, F., Mishra, P., Smyth, D.J., Wu, W., Lemenze, A., Kumamoto, Y., Maizels, R.M., Gause, W....
After transfection of Piezo1 and GCaMP6s into HEK293 cells, 100 μM GdCl3or LanND-Gd was added to the culture medium and co-incubated with cells for 24 h. Then, the cells were transferred to a chamber on the inverted fluorescence microscope (Ti-U, Nikon, Japan), and the bath ...