We first examined the cellular localization and brightness of G-Flamp1 in HEK293T cells. G-Flamp1, like Flamindo2 and Pink Flamindo, was evenly distributed in cytoplasm and nucleus (Fig.2aand Supplementary Fig.1b. The detailed imaging conditions throughout the paper are summarized in Supplem...
For cAMPFIRE-L, -M, and -H, respectively, n = 3, 4 and 4 beads; Hill coefficients = 0.65, 0.68, and 0.82. Source data Extended Data Fig. 7 Transfection and imaging of L2/3 neurons in the somatosensory cortex. a, Example brain slice images (representative of 21 mice) from a ...
GECIs with linear fluorescence responses to AP trains provide a large effective dynamic range for quantifying spike rates and facilitate counting spikes within trains. In purified protein, Hill coefficients were lower for the jGCaMP8 variants (1.9–2.2) than jGCaMP7f (3.1) (Extended Data Table3)...
Fig. 2.Chordotonal organs relevant to the detection of substrate vibrations. (A) Overview of the insect body plan indicating confirmed vibration-sensitive chordotonal organs (green circles) and campaniform sensilla (magenta squares). (B) The femoral chordotonal organ ofLocusta migratoria, midleg, ...
Heart Center Brandenburg, Department of Cardiovascular Surgery, University Hospital Brandenburg, Medical School Theodor Fontane, Ladeburger Straße 17, 16321 Bernau bei Berlin, Germany * Author to whom correspondence should be addressed. Cells 2022, 11(2), 308; https://doi.org/10.3390/cells1102030...
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Adeno-associated virus (AAV) encoding the fluorescent protein EYFP (AAV5-EF1a-DIO-EYFP) was obtained from the Vector Core at the University of North Carolina at Chapel Hill (UNC Vector Core, USA). AAV encoding GNAO1 variants (AAV9-Syn-DIO-Gαo-IRES-mCherry) were obtained from Vector...
5) While we’re there, and while there’s always couching when it comes to comparing any receiver to Tyreek Hill, Raiders folks talked about second-year man Tre Tucker like he has that kind of explosiveness and could add that sort of element to their offense. ...
Imaging was performed in the University of Colorado Anschutz Medical Campus Advanced Light Microscopy Core supported in part by NIH/NCATS Colorado CTSI Grant Number UL1 TR001082. H.E.A. would like to thank John Thompson, PhD, for his assistance in statistics. ...