Protocol: 1. Remove and discard culture medium. 2. add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove. . 3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 minutes). 4. Add...
5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. lncubate cultures at 37℃.操作流程1.吸走多余培养基,加入3-5mL PBS后轻轻晃动培养瓶润洗细胞; 2.吸干净PBS后加入1mL 0.25%胰酶-0.53mM EDTA,轻轻摇晃培养皿使胰酶浸没细胞表面,培养瓶放37度培养箱消化; (细胞对于消化比较敏...
5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. lncubate cultures at 37℃.操作流程1.吸走多余培养基,加入3-5mL PBS后轻轻晃动培养瓶润洗细胞; 2.吸干净PBS后加入1mL 0.25%胰酶-0.53mM EDTA,轻轻摇晃培养皿使胰酶浸没细胞表面,培养瓶放37度培养箱消化; (细胞对于消化比较敏...
To assess cell viability and ROS formation, 4 × 104cells were seeded in 3 ml complete medium. After 72 h of cell growth, culture medium was replaced with fresh medium and cell cultures were exposed/sham exposed to 370 mT SMF for 1 h/day for 4 consecutive days. Cells were...
5. Add appropriate aliquots of the cell suspension to new culture vessels. 6. lncubate cultures at 37℃.操作流程1.吸走多余培养基,加入3-5mL PBS后轻轻晃动培养瓶润洗细胞; 2.吸干净PBS后加入1mL 0.25%胰酶-0.53mM EDTA,轻轻摇晃培养皿使胰酶浸没细胞表面,培养瓶放37度培养箱消化; (细胞对于消化比较敏...
The cell viability was measured using the MTT Cell Viability Assay Kit (#30006, Biotium, Inc., Fremont, CA, USA) according to the manufacturer's protocol. The absorbance signal was measured using a spectrophotometer BioTek Cytation 5 Cell Imaging Multimode ReaderCytation (Agilent Technologies, ...
and 48 h at 37 °C. The cell viability was measured using the MTT Cell Viability Assay Kit (#30006, Biotium, Inc., Fremont, CA, USA) according to the manufacturer’s protocol. The absorbance signal was measured using a spectrophotometer BioTek Cytation 5 Cell Imaging Multimode ReaderCytation...
and 48 h at 37 °C. The cell viability was measured using the MTT Cell Viability Assay Kit (#30006, Biotium, Inc., Fremont, CA, USA) according to the manufacturer’s protocol. The absorbance signal was measured using a spectrophotometer BioTek Cytation 5 Cell Imaging Multimode ReaderCytation...