Real-time PCR quantification of gene expression in embryonic mouse tissue. Villalon E,Schulz D J,Waters S. T. Secondary Real-time PCR quantification of gene expression in embryonic mouse tissue . 2014Villalon E,Schulz D J,Waters S. T.Real-time PCR quantification of gene expression in ...
We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not...
samples (cell types). Here we found that this fraction is on AVG 0.81 in human cell lines, and 0.82 in mouse tissues—indicating that overall regulation of gene expression plays the major role in defining cell type specificity. This is consistent with tissue-dominated clustering of gene expressi...
We also compared the tissue specific expression patterns between mouse and rat for a subset of genes with high sequence conservation with respect to human. Accordingly, many highly conserved genes also have highly correlated gene expression patterns. In addition, the observed tissue specificity of ...
Furthermore, CR is thought to oppose age-associated gene expression patterns, but detailed statistical investigations of this hypothesis have not been carried out./p pResults/p pSystemic effects of CR and aging were identified by examining transcriptional responses to CR in 17 mouse tissue types, ...
We also found characteristic protein expression of immune-privileged organs, which may help understanding possible immune rejection after organ transplantation. Each tissue type expressed characteristic high-abundance proteins related to its physiological functions. We also uncovered some tissue-specific ...
In order to obtain valid data about the tissue specific expression it can be important and helpful to test gene expression with several independent techniques. Unfortunately, the current expression studies in mammals are heterogeneous with regard to species, applied methods, number of included tissues ...
Furthermore, software-based selection approaches rank reference genes differently and do not always identify genes which differ between conditions. Therefore, we recommend that reference gene selection for gene expression studies should be thoroughly validated for each tissue of interest. 展开 ...
Tissue-specific regulation of a chicken delta-crystallin gene in mouse cells: involvement of the 5' end region. Tissue- specific expression of a cloned chick S-crystallingene in mouse cells. Nature, 301, 440-442.Hayashi, S., H. Kondoh, K. Yasuda, G. Soma, Y. Ikawa and T.S. Okada:...
Large-scale quantitative analysis of transcriptional co-expression has been used to dissect regulatory networks and to predict the functions of new genes discovered by genome sequencing in model organisms such as yeast. Although the idea that tissue-spec