Inferring causality from time-series data poses considerable challenges; conventional methods of network reconstruction offer a static characterization of the network topologies. For example, correlation-based methods [5,6], matrix-based methods such as least-squares, principal component regression (PCR) ...
Murine lung epithelial 12 cells (MLE12; ATCC® CRL-2110) were maintained in DMEM/F12 1:1 media including HEPES (HyClone; SH30261) with the addition of 2% fetal bovine serum (FBS; HyClone; SH30088), insulin (0.005 mg per mL)-transferrin (0.01 mg per mL)-selenium 30 nM (...
149. Ly T, Ahmad Y, Shlien A, Soroka D, Mills A, Emanuele MJ, et al. A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells. Elife. 2014;3:e01630. 150. Cooper GM, Hausman RE. The cell: molecular approach: Medicinska naklada; 2004. 151. ...
To generate SARS-CoV-2 viral particles Vero E6 (ATCC# CRL-1586) cell monolayers were maintained in DMEM (Sigma-Aldrich; D5671) supplemented with 10% heat inactivated fetal bovine serum (Cytiva; sh30910.03). Flasks were inoculated with a dilution of 1:100 of SARS-CoV-2 USA-WA1/2020 isola...
Genetic deletion ofTrem2exerted different outcomes depending on the transgenic line, timing of the intervention and the age of the mouse [15]. Constitutive deletion in APP/PS1 mice led to decreased amyloid accumulation [15]. Knockdown ofTrem2at the early-to-middle stage of amyloid accumulation ...
(1:300, Cat# 71-5800, Lot# SH257822; Invitrogen, Waltham, MA). Primary antibodies for another series included rat anti-glial fibrillary acidic protein (1:2500, AB5804, Millipore, Lot # TA265137) or goat anti-Iba1 (1:1000, PA5-18,039, Lot #TI2638761, SJ2467805; ThermoFisher, ...
15. Park YK, Franklin JL, Settle SH, Levy SE, Chung E, Jeyakumar LH, Shyr Y, Washington MK, Whitehead RH, Aronow BJ, Coffey RJ: Gene expression profile analysis of mouse colon embryonic development. Genesis 2005, 41:1-12. 16. Reichling T, Goss KH, Carson DJ, Holdcraft RW, Ley-...
Biochemical networks are often described through static or time-averaged measurements of the component macromolecules. Temporal variation in these components plays an important role in both describing the dynamical nature of the network as well as provid
Longitudinal Evaluation of Familial Frontotemporal Dementia Subjects (LEF- FTDS) Study was made possible through the support of the U.S Department of Health and Human Services (DHHS) and the National Institute on Aging (NIA)/National Institute of Neurological Disorders and Stroke (NINDS) Grant U01...
(Fig.2c, Supplementary Fig.5c–e). We then investigated the functional impact of genetic reduction of MAP1B in ASD patient iPSC-derived neurons using lentivirus expressing shRNA (LV-shMAP1B) (Supplementary Fig.5f, g). Western blot analysis revealed that the MAP1B protein levels were ...