Given that mice deficient for MMR (for example, mutations in Mlh1 and Pms2) are infertile45,46,47, an immediate concern was that transient expression of mMLH1dn could impact fertility. We therefore microinjected mMLH1dn mRNA into two-cell-stage embryos, transferred these embryos into pseudo...
76. In previous retrotransposition assays conducted in cultured HeLa cells, 3TC was tested at a maximum concentration of 25 µM against the codon-optimized L1SM element, reducing its mobility by ~50%75. By performing
405-410 Google Scholar [13] H. Azuma, N. Paulk, A. Ranade, C. Dorrell, M. Al-Dhalimy, E. Ellis, et al. Robust expansion of human hepatocytes in Fah-/-/Rag2-/-/Il2rg-/- mice Nat. Biotechnol., 25 (2007), pp. 903-910 Google Scholar [14] Kd Bissig, Tt Le, Nb Woods, ...
During this incubation pinetroioadh, tuhmeiidmifmieodb3i7liz°eCdCaOn2tiibncoudbyaintotrhfeorima mspeedciiafiteedvpiceinriiotyd of of the secreting cells binds secreted TNF-α. After washing away any cells and unbound substances, a biotinylated polyclonal antibody specific for mouse TNF-α is ...
along with injection of autologous human CD34+ hematopoietic stem cells. In representative images, we show the engrafted human lymphoid tissues (human thymus xenograft-thymus, white tissue; human spleen xenograft-Spleen, dark brown tissue and the reconstituted rodent-spleen (humanized spleen-hSpleen)...
(K–M) Quantification of (K) cortical, (L) thalamic, and (M) hippocampal galectin-3 immunoblot normalized to H3. Data are presented as means ± SD. n = 5 mice/group. p values were calculated by one-way or two-way (D and I) ANOVA with Tukey’s post hoc analysis. ∗p < 0.05...
(NBP1-87726 and NB120-6588, Novus Biologicals) at 4 °C under gentle shaking. Subsequently, membranes were incubated at RT for 1 h with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies (NA9310V and NA9340V respectively, Ge Healthcare) followed by chemiluminescence reaction ...
S1), overnight-fasted mice from ON-W, ON-C, ON-CC, ON-NB groups lost 13–14% body weight (Fig. 1b) while ON-G mice lost 16% body weight suggesting the impact of coprophagy and/or cage bedding consumption on body weight. CR mice lost 20–22% body weight within 14 days with ...
Schiller NB, Shah PM, Crawford M, De Maria A, Devereux R, Feigenbaum H et al. Recommendations for quantitation of the left ventricle by two-dimensional echocardiography American Society of Echocardiography Committee on Standards, subcommittee on Quantitation of Two Dimensional Echocardiograms. J Am So...
H&E shown in the top panels. Bottom panels show immunohistochemistry (IHC) labeling against SARS-CoV-2 nucleocapsid, counterstained with hematoxylin. Scale bars represent 200 μm. Error bars represent SEM about the mean. ∗p < 0.05. SARS-CoV-2 MA10 Cellular Tropism To characterize the ...