Animal models provide preclinical tools to investigate the causal role of genetic mutations and environmental factors in the etiology of autism spectrum disorder (ASD). Knockout and humanized knock-in mice, and more recently knockout rats, have been gene
Stephen H Settle†, Braden Boone†, Shawn Levy†, Jonathan M Graff§§, Thomas Doetschman#, Joanna Groden¥, William F Dove‡, David W Threadgill§, Timothy J Yeatman††, Robert J Coffey Jr† and Bruce J Aronow* Addresses: *Biomedical Informatics, Cincinnati Children's Hospit...
Massively parallel sequencing of mRNA isoforms has led to a rapid accumulation of expression and sequence data at the mRNA isoform level. RNA-Seq has provided evidence confirming the production and expression of distinct mRNA isoforms under different conditions9,16,17. This has led to the improvemen...
The radiotracer was also shown to be stable for more than 10 h. Then, the aliquot was mixed with nonradioactive 1 standard solution and injected into the same HPLC system. The result of the co-injection confirms that the radioactive peak was eluted together with reference standard 1. (...
Genes encoding Toll-like receptors (TLRs) are expressed by germ cells in the mouse testis. Nevertheless, the expression of TLRs by germ cells has only been
(d) MT1-MMP assay with bispecific FAF Nb-MSA21 nanobodies. Lane 1; negative control without MT1-MMP; lane 2: positive control with MT1-MMP but without addition of a nanobody. Lanes 3–4 represent additional controls in which an equimolar amount of either albumin or nanobody was added. ...
For instance, following novel context exploration we observed no decrease in tCREB levels in Tg mice using either immunohistochemistry (Figures S1c and S1d) or western blot analysis (Figures 1g and h; significant effects of Genotype  CREB (tCREB, pCREB) po0.05), (F1,6 ¼ 18.04; and...
Finally, we examined a known fraction of the total tissue thickness using the confocal microscope (tsf) defined as the average height of imaging depth per mouse (h) divided by the total tis- sue thickness (t) accounting for shrinkage with process- ing, where h varied per mouse (range: 9...
Therefore, we used the 40-day control group for behavioral and further memory dysfunction. Treatment molecular analyses. The with osmotin (15 μ g/g, ri.eps.u, lt4s hsru)gsgiegsnteifdictahnattlyAiβn 1c-r42eainsejedctsipoonnitnadnueoceuds (aplt e...
The intestinal microbiota modulates host physiology and gene expression via mechanisms that are not fully understood. Here we examine whether host epitranscriptomic marks are affected by the gut microbiota. We use methylated RNA-immunoprecipitation and s