For research use only. The Mouse Direct PCR Kit provides a fast preparation and PCRamplification that is specifically designed for mouse genotyping. Selleck's has been cited by68publications Cell Discov,20239(1):69 Immunity,202154(8):1807-1824.e14 ...
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For standard genotyping, mouse ear-punch tissue samples were digested at 55uC for 3 h in 25–100 ml of a lysis buffer containing 10% (w/v) sodium dodecyl sulphate and with 2 mg/ml proteinase K (Sigma). 1 ml of a 1510 dilution of each sample was used for genotyping by PCR in a ...
Thus, hCD147KIhet-NSG mice were used for the purpose of evaluating SARS-CoV-2 sensitivity. Fig. 1 Schematic representation of genotyping primers used to confirm hCD147KI expression. A combination of 4 primers was used to screen mice (internal primers hCD147A and hCD147B) and confirm ...
The mouse gDNA was extracted from mouse toe or ear samples using the KAPA Mouse Genotyping Kit. Briefly, tissues around 2 mm3 were digestion in a 100 ml volume containing 88 ml PCR-grade H2O, 2 ml 103 KAPA express Extract buffer and 2 ml KAPA Express Extract Enzyme, at 75uC for 15 ...
Genotyping was done on genomic DNA from the tail or ear, using standard PCR amplification and gel electrophoresis. The DNA primers (Sigma-Aldrich) used for genotyping are listed in Additional File 1: Table S7. During the treatment period, 5-mo. AppNL-G-F mice received a daily ...
Brain sampling was performed indiscriminately once pups grew up to the appropriate age, and only brains of the desired genotype were later selected by PCR genotyping of the reserved tissues for CreER internal sequences. At the time of sampling, the sex of the mice was still obscure and ...
Conditional knock out mouse genotyping Mouse tail was collected after weaning, and lysed in 75 μl lysis buffer (0.07% NaOH, 0.7 mM EDTA) at 95°C for 30 min before adding 75 μl neutralization buffer (40 mM Tris-HCl, pH 5.0), and stored at 4°C. Then, the genotype was confirmed ...
(b) Representative data on genotyping PCR of offspring from transgenic rescue experiments using tail DNA (line-458). Positions of primer pairs (x, y and z) are indicated in a. The C57BL/6-specific sequence (asterisk in a) was detected with a primer pair z to distinguish the WT allele ...
Age-related macular degeneration (AMD) is the leading cause of blindness among the elderly. Dry AMD has unclear etiology and no treatment. Lipid-rich drusen are the hallmark of dry AMD. An AMD mouse model and insights into drusenogenesis are keys to bett