To facilitate the use of our single-cell datasets, an online tool was generated for evaluation of gene expression at single-cell resolution:www.brainimmuneatlas.org. All gene–cell count matrices can also be downloaded via this link. In addition, all scRNA-seq and bulk RNA-seq data are also...
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Cells were plated in 10 cm dish, grown to 70% confluence and treated with Colchicine in culture at 1.0 μg/mL for primary liver cancer cell line, 0.8 μg/mL for NIH-3T3 and Hepa1-6 cells and cells for 6 h. Cells were then harvested with 0.25% trypsin, resuspended in 75 mM KCl ...
2020). We have imported these TPM level data from the Expression Atlas for those RNA-seq experiments that are within GXD’s scope (as determined by our indexing work described above). We have processed these data further to enable their full integration into GXD. Taking advantage of our ...
Therefore, we proposed to create a cell atlas of the whole tooth germ in varied development stages in single cell resolution. Although advances in single cell RNA sequencing (scRNA-seq) technology have provided insights into cell heterogeneity during embryogenesis in vivo [20], [21], [22], [...
with UMAP visualization of cells colored by expression of two isoforms that have differential expression between quiescent and activatedMuSC.IscATAC-seq read coverage for PRDX1 with cells from each cell line aggregated and plotted together. UMAP plots showing isoform expression, with each cell colored...
We have used a number of banding techniques to analyze the chromosomal content of two sublines of the steroid-secreting mouse adrenocortical tumor cell line Y1. One of the sublines contains marker chromosomes with large "homogeneously staining regions" (HSRs). The other subline contains numerous ...
This breast cancer-specific activation of FAS-dependent lipogenesis, an anabolic-energy-storage pathway of minor importance in normal cells, would render ... J Menendez,I Mehmi,E Atlas,... - 《International Journal of Oncology》 被引量: 235发表: 2004年 Insulin-like growth factor-1 induces lip...
the single-cell ovarian aging atlas presented herein serves as a crucial resource for the field. In nonhuman primates, scRNA-seq has provided mechanistic insights into changes associated with ovarian aging18. However, this study was conducted with animals in the perimenopausal state78when alterations ...
(CPM) centered expression values in each lineage. The number of lineage-specific genes shown is indicated on the left, up to 200 genes are shown per lineage. For each lineage, 8 genes with the highest expression in the respective cell line are labeled.cLineage-specific gene expression ...