The resulting dual-AAV systems, v1em and v3em PE-AAV, enable therapeutically relevant prime editing in mouse brain (up to 42% efficiency in cortex), liver (up to 46%) and heart (up to 11%). We apply these systems to install putative protective mutations in vivo for Alzheimer’s ...
小鼠脑成纤维细胞提取物Mouse Brain: Normal Brain Fibroblasts Derivatives对于贴壁细胞,传代可参考以下方法: 1.弃去培养上清,用不含钙、镁离子的PBS润洗细胞1-2次。 2.加2ml消化液(0.25%Trypsin-0.53mM EDTA)于培养瓶中,置于37℃培养箱中消化1-2分钟,然后在显微镜下观察Ⅱ型肺泡上皮细胞|细胞消化情况,若细胞大...
-removed mice were rested for at least 24 h in a cage, and intraperitoneally injected with cDNAs encoding CAG-Magnets, CAG-Magnets-opti, Cre, or pCAG-Flpe (Addgene, #13787) using TransIT®-EE Delivery Solution (Mirus Bio LLC), respectively, according to the manufacturer’s protocol. ...
Blood–brain barrier damage is a critical pathological feature of ischemic stroke. Oligodendrocyte precursor cells are involved in maintaining blood–brain barrier integrity during the development. However, whether oligodendrocyte precursor cell could sustain blood–brain barrier permeability during ischemic brai...
Table 5C). To interrogate the reliability of our findings, we performed western blot analysis in a separate CSDS cohort for the only protein affected in all three brain regions and the top affected protein in the NAc, RPRD2 (regulation of nuclear pre-mRNA domain containing 2), which plays ...
Western Blotting The mice were sacrificed on the days of 1, 4 and 7 after the last MPTP injection. The brain tissues were removed and the SNpc were quickly dissected on the ice. The tissues were homogenized in protein lysis buffer which composed by 150 mM NaCl, 5 mM EDTA, 50 mM...
Neurodegenerative diseases such as Alzheimer’s disease are characterized by the progressive spreading and accumulation of hyper-phosphorylated tau protein in the brain. Anti-tau antibodies have been shown to reduce tau pathology in in vivo models and an
We selected PKCα based on a combination of our experience characterizing PKCα as an mTORC2 target in the brain14, the availability of antibodies for Western blot analyses and because CRISPR has been used to successfully eliminate PKCα in the retina15. As low PKCα levels, due to loss of...
Glutathione Peroxidase 1 Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请...
Alternative splicing (AS) is involved in cell fate decisions and embryonic development. However, regulation of these processes is poorly understood. Here, we have identified the serine threonine kinase receptor-associated protein (STRAP) as a putative sp