While the manual approach is limited to small regions-of-interest in the brain, automated tools are needed to up-scale translational approaches and generate whole mouse brain counts in an atlas framework. Our goal was to develop an algorithm which requires no pre-training such as machine ...
The mouse brain atlas was quoted from ref. [42]. F The three-dimensional (3D) brain model, based on a C57BL/6 mouse brain atlas built from MRI and Nissl histology, which consists of 39 different brain segments (in different colors, F1). F2–F4 The top (F2), front (F3), and ...
Probe location was checked post hoc by aligning it to the Allen Mouse Brain Atlas41 visually or through custom software (www.github.com/petersaj/AP_histology). Before and in between experiments, mice were housed in individually ventilated cages (IVC), with a 9 am light/9 pm dark cycle...
We use a digital atlas as the hub of an informatics network, correlating imaging data, such as structural imaging and histology, with text-based data, such as nomenclature, connections, and references. We generated brain volumes using magnetic resonance microscopy (MRM), classical histology, and ...
The new edition of The Embryonic Human Brain: An Atlas of Developmental Stages represents the integration of analysis of the serial sections of human embry... R O'Rahilly,F Müller - Wiley-Liss 被引量: 195发表: 2005年 High-resolution magnetic resonance histology of the embryonic and neonatal ...
Illustration modified from the coronal section 0.98 mm anterior to bregma in mouse brain atlas of Franklin and Paxinos (1997). aCSF, artificial cerebrospinal fluid; DMS, dorsomedial striatum; VMS, ventromedial striatum. mice treated with 0.3 mg/kg showed a trend toward social preference (p ¼ ...
The Allen Mouse Brain Atlas (Allen Institute for Brain Science, 2019) was used as a guide to identify brain structures and draw boundaries. Regions of interest were manually outlined in each section and converted into area values (mm2) with Zeiss ZEN imaging software. The volumes of the ...
Immuno-fluorescence-histochemistry Coronal brain sections (30 μm) containing the hippocam- pus were cut on a cryostat (CM1950, Leica, Wetzlar, Germany) using the Mouse Brain Atlas as a reference [27]. Free-floating brain sections were kept in a cryoprotectant solution (30% glycerol, 30% ...
In order to assess this clearance from the brain, we injected 2 μL of the undiluted PFC emulsion in the striatum of naïve mice (n = 3). Animals were imaged with 19F-MRI and sacrificed for histology at different time points. After four weeks, 55% of the initial 19F signal at 24 ...
"Maps are a fundamental infrastructure to build knowledge upon but we don't have a high-resolution 3D atlas of the developing brain," said Yongsoo Kim, associate professor of neural and behavioral sciences at Penn State College of Medicine and senior author on the paper. "We are generating h...