Monoclonal Anti-Iba1 antibody produced in mouse IRT-1 IRT1 IBA1 AIF-1 基本信息 NACRES NA.41 General description【一般描述】 离子钙结合衔接子分子1(IBA1),又称同种异体移植炎症因子1(AIF1),定位于人类染色体 6p21.33。其编码分子量为 17 kDa的蛋白,包含与钙结合的EF手基序。IBA1 在肺、扁桃体、淋巴...
Anti-Iba1/AIF1 Antibody clone 20A12.1, from mouse; Synonyms: Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1 at Sigma-Aldrich
Anti-Iba1/ AIF1 Antibody, clone 20A12.1 ZooMAb® Mouse Monoclonal recombinant, expressed in HEK 293 cells; Synonyms: Allograft inflammatory factor 1,Ionized calcium-binding adapter molecule 1,Protein G1; find Sigma-Aldrich-ZMS1024 MSDS, related peer-re
common markers for microglial visualization, was performed as previously described [64]. While Iba1, a cytoplasmatic protein responsible for cytoskeletal reorganization, is also expressed by peripheral macrophages; TMEM119 is an important regulator of the Wnt/β-catenin...
The following primary antibodies were used: anti-IBA1 antibody (rabbit polyclonal, Wako-01919741 1 : 1000) and anti-GFAP antibody (rabbit polyclonal, ab7260 1 : 500). Sections were rinsed with 1× PBS and then incubated with secondary antibody (488 Alexa Fluor donkey anti-rabbit ...
fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba1) with antibodies mouse anti-GFAP (catalog# 837,202, BioLegend) and rabbit anti-IBA-1 (catalog# WDG5619, WAKO) each at a dilution of 1:1000, followed by secondary antibodies and peroxidase development [35]...
Cells were stained with DAPI (1:10′000) for 10 min, mounted on object slides and embedded in Mowiol 4–88 (Roth). The following antibodies and concentrations were used: anti-CD77 (rat monoclonal, Beckman Coulter IM0175, 1:100), anti-IBA1 (goat polyclonal, Novus Biologicals NB100-...
Retinal whole-mounts were immunostained with anti-Iba1. In Matlab, new algorithms of segmentation and control of distances were developed to determine the number of Iba-1+ cells. The automatic results were compared with those from direct human observation of the images.Results The algorithm method...
After a hydrogen peroxide treatment and blocking serum, the sections were immunostained with the primary antibodies (i.e anti-Iba1, anti-GFAP), and incubated overnight at room temperature. Vehicle solutions contained Triton X-100 for permeabilization. Following rinses, a biotinylated secondary ...
The sections were incubated at 4℃ with primary antibodies including anti-Iba-1 (#MA5-41239, 1:500, Thermo Fisher Scientific), antibody against Neurofilament (#ab204893, 1:500, Abcam, USA), anti-RPS27A (#ab227011, 1:500, Abcam) and anti-Ly6G (#12-9668-82, Invitrogen). The next ...