Monoclonal Anti-Iba1 antibody produced in mouse IRT-1 IRT1 IBA1 AIF-1 基本信息 NACRES NA.41 General description【一般描述】 离子钙结合衔接子分子1(IBA1),又称同种异体移植炎症因子1(AIF1),定位于人类染色体 6p21.33。其编码分子量为 17 kDa的蛋白,包含与钙结合的EF手基序。IBA1 在肺、扁桃体、淋巴...
Anti -Iba1 Mouse mAb,ProductInformationDescriptionIba1mousemonoclonalantibodyProteinfullnameAllograftinflammatoryfactor1SynonymsAIF1,AIF-1,IBA,IRT-1,IRT1,allograftImmunogenRecombinantproteincorrespondingtoMouseIba1Isotype
Anti-Iba1/AIF1 Antibody clone 20A12.1, from mouse; Synonyms: Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1 at Sigma-Aldrich
Monoclonal Anti-Iba1 antibody produced in mouse (AIF-1 ); monoclonal GT10312, affinity isolated antibody; Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections); Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, I
mouse-anti-IBA1 (1:1000, Abcam, Ab178847), rabbit-anti-CD68 (1:100, Novus Biologicals, NB100-683) and mouse-anti-6E10 (1:1000, ITK Diagnostics, SIG-39320) and for morphometrics analysis rabbit-anti-IBA1 (1:1000, WAKO, 019–19741 in 0.1 % Triton-X and 1 % normal donkey serum)...
The following primary antibodies were used: anti-IBA1 antibody (rabbit polyclonal, Wako-01919741 1 : 1000) and anti-GFAP antibody (rabbit polyclonal, ab7260 1 : 500). Sections were rinsed with 1× PBS and then incubated with secondary antibody (488 Alexa Fluor donkey anti-rabbit ...
0.5 µM 647-labelled Aβ was added and after 24 h cells were fixed and counterstained with anti-IBA1 (Wako 019-19741, 1:1000). Quantification of Z-stacks taken at the confocal microscope with constant settings was performed with Image J: a mask was created for each IBA1 stained cell...
在本研究中,我们染色星形细胞培养与小胶质细胞特异性标记IBA1或神经元标记MAP2,以便确定任何小胶质细胞或神经元的潜在污染物的存在。在啮齿动物星形胶质细胞培养小胶质细胞的比例可以根据若干因素,包括动物的年龄,种类,地区,培养基,传代培养法,振荡30变化为1%至30%。在我们的文化中,我们观察到小神经胶质细胞污染的...
Unspecific binding was blocked by incubation with 5% BSA for 1 h at room temperature. Incubation with primary antibody against IBA1, OCT4, was conducted overnight at 4 °C. Secondary anti-rabbit antibodies coupled to Alexa Fluor 488 (Invitrogen, A11034) or 568, (Invitrogen, A10037) ...
The sections were incubated at 4℃ with primary antibodies including anti-Iba-1 (#MA5-41239, 1:500, Thermo Fisher Scientific), antibody against Neurofilament (#ab204893, 1:500, Abcam, USA), anti-RPS27A (#ab227011, 1:500, Abcam) and anti-Ly6G (#12-9668-82, Invitrogen). The next ...