Monoclonal Anti-Iba1 antibody produced in mouse IRT-1 IRT1 IBA1 AIF-1 基本信息 NACRES NA.41 General description【一般描述】 离子钙结合衔接子分子1(IBA1),又称同种异体移植炎症因子1(AIF1),定位于人类染色体 6p21.33。其编码分子量为 17 kDa的蛋白,包含与钙结合的EF手基序。IBA1 在肺、扁桃体、淋巴...
Anti -Iba1 Mouse mAb,ProductInformationDescriptionIba1mousemonoclonalantibodyProteinfullnameAllograftinflammatoryfactor1SynonymsAIF1,AIF-1,IBA,IRT-1,IRT1,allograftImmunogenRecombinantproteincorrespondingtoMouseIba1Isotype
Anti-Iba1/AIF1 Antibody clone 20A12.1, from mouse; Synonyms: Allograft inflammatory factor 1, AIF-1, Ionized calcium-binding adapter molecule 1, Protein G1 at Sigma-Aldrich
Monoclonal Anti-Iba1 antibody produced in mouse (AIF-1 ); monoclonal GT10312, affinity isolated antibody; Suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections); Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, I
For morphometrics analysis biotinylated donkey-anti-rabbit (1:400, Jackson ImmunoResearch, 711–065-152) was used followed by incubation in ABC solution at RT for 30 min (VECTASTAIN® ABC Kit, Vector Laboratories, PK-6100) and finally visualization of IBA1-immunoreactivity by 3,3′-...
TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A sing...
在本研究中,我们染色星形细胞培养与小胶质细胞特异性标记IBA1或神经元标记MAP2,以便确定任何小胶质细胞或神经元的潜在污染物的存在。在啮齿动物星形胶质细胞培养小胶质细胞的比例可以根据若干因素,包括动物的年龄,种类,地区,培养基,传代培养法,振荡30变化为1%至30%。在我们的文化中,我们观察到小神经胶质细胞污染的...
The following primary antibodies were used: anti-IBA1 antibody (rabbit polyclonal, Wako-01919741 1 : 1000) and anti-GFAP antibody (rabbit polyclonal, ab7260 1 : 500). Sections were rinsed with 1× PBS and then incubated with secondary antibody (488 Alexa Fluor donkey anti-rabbit ...
Unspecific binding was blocked by incubation with 5% BSA for 1 h at room temperature. Incubation with primary antibody against IBA1, OCT4, was conducted overnight at 4 °C. Secondary anti-rabbit antibodies coupled to Alexa Fluor 488 (Invitrogen, A11034) or 568, (Invitrogen, A10037) ...
Computerised reconstructions of immunostained sections map the extent of the neuronal damage (loss of MAP-2 or NeuN signal) and its spatial relation with post-stroke glial (GFAP for astrocytes, Iba1 for microglia, MBP-1 for oligodendrocytes) and vascular (CD31 for endothelial cells) reactions. ...