To compare laminar positioning in M1 and MTG, we estimated the relative cortical depth from pia for each neuron on the basis of layer dissection and average layer thickness46. Integrating Cv3 snRNA-seq datasets across species To identify homologous cell types across species, we used Seurat’s ...
The establishment of functional neuromuscular circuits relies on precise connections between developing motor axons and target muscles. Motor neurons ...
2). However, one cannot exclude some minor, microscopical changes in subcortical structures, as indeed found with the large number of vesicles observed in RNm neurones. The soma size of SMI-32 positive neurons in the RNm of the PMG monkey was however in the same range of that reported for...
Sp1-regulated expression of p11 contributes to motor neuron degeneration by membrane insertion of TASK1 Abstract Disruption in membrane excitability contributes to malfunction and differential vulnerability of specific neuronal subpopulations in a number of neurological diseases. The adaptor protein p11, and ...
There are significant differences in pattern modulation by exogenous serotonin compared to increased endogenous serotonin A, schematic diagram of the isolated brainstem–spinal cord preparation. The horizontal line labelled 'split' represents the location of the petroleum jelly barrier which isolates the ...
The term motor neuron disease encompasses a spectrum of disorders in which motor neurons are the lost. Importantly, while some motor neurons are lost early in disease and others remain intact at disease end-stage. This creates a valuable experimental paradigm to investigate the factors that regulate...
The floccular lobe is part of a loop that is superimposed on the basic pathways mediating the VOR and probably also the OKR, and in addition, it probably also serves as an output control system for SP. The three-neuron reflex path of the VOR consists of the ganglion cells of the vestibu...
Flow chambers (∼10 μl volume) were constructed by attaching clean #1.5 cover glasses to microscope slides using double-sided adhesive tape. Microtubules were polymerized from a mixture of HiLyte647-labelled and unlabelled tubulins (Cytoskeleton) in BRB80 buffer (80 mM PIPES/KOH, pH 6.8...
schematic showing classification of cortical projection neuron types based on their laminar positions, projection neuron class (IT, PT and CT), and specific projection targets. Right (top), analysis of the MOp-ul layer organization by hierarchical clustering of soma depth for retrogradely labelled ...
Microtubules were polymerized from a mixture of HiLyte647-labelled and unlabelled tubulins (Cytoskeleton) in BRB80 buffer (80 mM PIPES/KOH, pH 6.8, 1 mM MgCl2, 1 mM EGTA) in the presence of 1 mM GTP and 4 mM MgCl2 at 37 °C and stabilized by the addition of 20 mM Taxol. ...